Team:BYU Provo/Notebook/Phage Purification/Springexp/Period1/Dailylog
From 2013.igem.org
(Difference between revisions)
(12 intermediate revisions not shown) | |||
Line 4: | Line 4: | ||
{| width="100%" | {| width="100%" | ||
- | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification May - June Notebook: May | + | | colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification May - June Notebook: May 1 - May 12 Daily Log'''</font> |
<br> | <br> | ||
Line 10: | Line 10: | ||
|- valign="top" | |- valign="top" | ||
- | | style="width: | + | | style="width: 20%; background-color: transparent;"| |
<font color="#333399" size="3" font face="Calibri"> | <font color="#333399" size="3" font face="Calibri"> | ||
- | : | + | <font size = "4"> |
+ | |||
+ | : <u> '''Phage Purification''' </u> </font> | ||
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]] | : [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]] | ||
Line 26: | Line 28: | ||
</font> | </font> | ||
- | |||
- | |||
- | + | | style="width: 80%; background-color: transparent;"| | |
+ | <font face="Calibri" size="3"> | ||
<br> | <br> | ||
+ | <html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period4/Dailylog"><< Previous</a> | ||
+ | |||
+ | <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period2/Dailylog" style="display:block;float:right;">Next >></a> | ||
+ | </html> | ||
- | < | + | <br> |
- | + | ||
+ | <font size="4"> '''5/1/13''' </font> | ||
+ | : Today we created a detailed tentative plan to follow for the rest of the semester. https://docs.google.com/document/d/1ffqtGWjGOqEuOgNd46yK38lzkzMVdoCgXPcxMcWfCcc/edit?usp=sharing We went through the protein purification procedure to figure out the times that each step would take. https://docs.google.com/document/d/1W56-4bIIKsWBDtxsY2VgwV1q5BTo2nIXmrOLuqA0U9A/edit?usp=sharing | ||
<br> | <br> | ||
- | <font size="4"> ''' | + | <font size="4"> '''5/3/13''' </font> |
- | + | : We created an Excel sheet to calculate the amounts of each reagent for the phage purification experiment we will be running on Monday. This will allow for quick and easy calculations of reagents needed each time we run the experiment. | |
- | + | <br> | |
- | + | <font size="4"> '''5/5/13''' </font> | |
+ | : Today we created fresh bacteria to work with | ||
+ | : [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.5 Bacteria Cultures, 25 mL |5.5 Bacteria Cultures, 25 mL]] | ||
+ | :In the results, W3110 failed to grow, so the experiment will have to be repeated. | ||
+ | : We also centrifuged bacterial lysate infected with phage in order to store the phage for future use. | ||
+ | : [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.6 Phage From Lysate |5.6 Phage From Lysate]] | ||
+ | : We are waiting on materials to begin running the phage purification experiments. | ||
<br> | <br> | ||
- | <font size="4"> ''' | + | <font size="4"> '''5/8/13''' </font> |
- | + | : New liquid cultures of both W3110 and BL21 were created following the steps in [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.8 Bacteria Cultures, 25 mL |5.8 Bacteria Cultures, 25 mL]] | |
- | + | : After 1 hour of growth, BL 21 had T7 phage added to allow 48 hours infection time. | |
- | : | + | : [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.8 Infect With Phage |5.8 Infect With Phage]] |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | : [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/ | + | |
- | + | ||
<br> | <br> | ||
- | <font size="4"> ''' | + | <font size="4"> '''5/10/13''' </font> |
- | + | :We made a W3110 liquid culture | |
+ | : [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.10 Bacteria Cultures, 25 mL |5.10 Bacteria Cultures, 25 mL]] | ||
+ | :We emailed Dr. McCleary on ultracentrifuge training. | ||
<br> | <br> | ||
- | |||
- | |||
<br> | <br> | ||
- | |||
- | |||
Line 78: | Line 82: | ||
- | < | + | </font> |
- | </ | + | <html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period4/Dailylog"><< Previous</a> |
+ | |||
+ | <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Springexp/Period2/Dailylog" style="display:block;float:right;">Next >></a> | ||
+ | </html> | ||
|} | |} | ||
{{TeamBYUProvoFooter}} | {{TeamBYUProvoFooter}} |
Latest revision as of 00:02, 28 September 2013
Phage Purification May - June Notebook: May 1 - May 12 Daily Log
| ||
|
5/1/13
5/3/13
5/5/13
5/8/13
5/10/13
|