Team:BYU Provo/Notebook/Phage Purification/Springexp/Period1/Dailylog
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+ | : <u> '''Phage Purification''' </u> </font> | ||
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]] | : [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]] | ||
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: Today we created fresh bacteria to work with | : Today we created fresh bacteria to work with | ||
: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.5 Bacteria Cultures, 25 mL |5.5 Bacteria Cultures, 25 mL]] | : [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.5 Bacteria Cultures, 25 mL |5.5 Bacteria Cultures, 25 mL]] | ||
+ | :In the results, W3110 failed to grow, so the experiment will have to be repeated. | ||
: We also centrifuged bacterial lysate infected with phage in order to store the phage for future use. | : We also centrifuged bacterial lysate infected with phage in order to store the phage for future use. | ||
: [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.6 Phage From Lysate |5.6 Phage From Lysate]] | : [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.6 Phage From Lysate |5.6 Phage From Lysate]] | ||
- | + | : We are waiting on materials to begin running the phage purification experiments. | |
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+ | <font size="4"> '''5/8/13''' </font> | ||
+ | : New liquid cultures of both W3110 and BL21 were created following the steps in [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.8 Bacteria Cultures, 25 mL |5.8 Bacteria Cultures, 25 mL]] | ||
+ | : After 1 hour of growth, BL 21 had T7 phage added to allow 48 hours infection time. | ||
+ | : [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.8 Infect With Phage |5.8 Infect With Phage]] | ||
<br> | <br> | ||
- | <font size="4"> ''' | + | <font size="4"> '''5/10/13''' </font> |
+ | :We made a W3110 liquid culture | ||
+ | : [[Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period1/Exp/5.10 Bacteria Cultures, 25 mL |5.10 Bacteria Cultures, 25 mL]] | ||
+ | :We emailed Dr. McCleary on ultracentrifuge training. | ||
+ | <br> | ||
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Latest revision as of 00:02, 28 September 2013
Phage Purification May - June Notebook: May 1 - May 12 Daily Log
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5/1/13
5/3/13
5/5/13
5/8/13
5/10/13
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