Team:Bielefeld-Germany/Biosafety

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==Motivation==
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==Overview==
[[File:IGEM Bielefeld 2013 Biosafety E.coli bewaffnet safe 2..png|250px|left|thumb]]
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Biosafety is an essential aspect when taking part in iGEM especially when you work with living organisms which could possibly get out of your application by damage or incorrect handling. It is important to protect the population and the environment by developing and applying systems which comply these aspects. In order to counter this problem there exist useful systems to prevent the bacteria from escaping or killing the bacteria when they are outside of the application. To complement this archive we constructed not only one system but also three systems which differ in leakiness and strength. For this approach we combined two common Biosafety-ideas, an auxotrophic and a toxic gene product, in one device. So the constructed Biosafety-System takes the best of this two approaches and is characterized by a double kill-swtich system. This double kill-switch mechanism provides additional a higher plasmid stability and a higher resistance towards undesirable mutations. In one sentence: Our Biosafety-System is safe!
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Biosafety is an essential aspect when taking part in iGEM, especially when you work with living organisms which could possibly get out of your application due to damage or incorrect handling. It is important to protect the population and the environment by developing and applying systems which address these aspects. There already exist several useful systems which prevent the bacteria from escaping or killing them outside a defined area. To complement this we constructed three systems which differ in leakiness and strength. In these Biosafety-Systems we combined two common Biosafety ideas, an auxotrophic strain and a toxic gene product, in one device.
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Thereby the constructed Biosafety system takes the best parts of these two approaches and is therefore characterized as a double kill-switch system. This double kill-switch mechanism provides higher plasmid stability and additionally higher resistance towards undesirable mutations. In short: Our [https://2013.igem.org/Team:Bielefeld-Germany/Biosafety/Biosafety_System Biosafety-System] is safe!  
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==Application==
==Application==
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The device we designed is everywhere applicable. Everywhere a closed system is used where E.coli is located to produce products which isn't allowed to get out. It can also be used to produce toxic products by induction with the right inducer. The alanince racemase which is in the device is a new opportunity to arrange selection like an antibiotic would do. With our device it isn’t necessary to supplement antibiotics to the culture media. Because of the fact that our whole MFC system within the safety strain is so safe and independent of weather it can be used in wastewater treatment, for small applications at home or in the Third World to gain electricity. For detailed information about using the MFC in actual and future application see our [https://2013.igem.org/Team:Bielefeld-Germany/Project/Applications Application] site.
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Biosafety of the MFC is based on several layers. First of all, the device we designed is a closed system, thus preventing the escape of ''E. coli'' into the environment. If the physical containment, i.e. the MFC, is breached, lack of proper inducers which should be absent in the environment, results in the production of a toxic protein. This toxic protein is called Barnase, a RNase of ''Bacillus amyloliquefaciens'' which degrades the RNA and causes the cell death. Finally, presence of a plasmid encoded alanine racemase in ''E. coli'' strains lacking a chromosomal copy of this enzyme enforces plasmid stability without the need of additional antibiotics. All in all this aspects make the individual systems very safe and usable outside of the laboratory. To assess the usability of our MFC regarding potential applications, we searched for a [https://2013.igem.org/Team:Bielefeld-Germany/Project/MFC_Efficiency#Feasibility_study_for_MFC_in_sewage_and_waste_treatment feasibility study] and compared it to our results. It shows, that our currently achieved efficiencies have the potential for efficient energy production in real world applications.
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==References==
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Not only the currently used applications are important but also the future applications. For detailed information about using the MFC in actual and future applications see our [https://2013.igem.org/Team:Bielefeld-Germany/Project/Applications Application] site.
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Latest revision as of 02:42, 29 October 2013



Biosafety


Overview

IGEM Bielefeld 2013 Biosafety E.coli bewaffnet safe 2..png

Biosafety is an essential aspect when taking part in iGEM, especially when you work with living organisms which could possibly get out of your application due to damage or incorrect handling. It is important to protect the population and the environment by developing and applying systems which address these aspects. There already exist several useful systems which prevent the bacteria from escaping or killing them outside a defined area. To complement this we constructed three systems which differ in leakiness and strength. In these Biosafety-Systems we combined two common Biosafety ideas, an auxotrophic strain and a toxic gene product, in one device. Thereby the constructed Biosafety system takes the best parts of these two approaches and is therefore characterized as a double kill-switch system. This double kill-switch mechanism provides higher plasmid stability and additionally higher resistance towards undesirable mutations. In short: Our Biosafety-System is safe!



Application

Biosafety of the MFC is based on several layers. First of all, the device we designed is a closed system, thus preventing the escape of E. coli into the environment. If the physical containment, i.e. the MFC, is breached, lack of proper inducers which should be absent in the environment, results in the production of a toxic protein. This toxic protein is called Barnase, a RNase of Bacillus amyloliquefaciens which degrades the RNA and causes the cell death. Finally, presence of a plasmid encoded alanine racemase in E. coli strains lacking a chromosomal copy of this enzyme enforces plasmid stability without the need of additional antibiotics. All in all this aspects make the individual systems very safe and usable outside of the laboratory. To assess the usability of our MFC regarding potential applications, we searched for a feasibility study and compared it to our results. It shows, that our currently achieved efficiencies have the potential for efficient energy production in real world applications.
Not only the currently used applications are important but also the future applications. For detailed information about using the MFC in actual and future applications see our Application site.









Contents