Team:Bielefeld-Germany/Labjournal/Analytics
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<br><b><u>Fluorescence measurements </u></b> | <br><b><u>Fluorescence measurements </u></b> | ||
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+ | Measuring of mRFP with Tecan Infinite® M200 platereader</p> | ||
- | + | <p>Take at least 500 µL sample for each measurement (200 µL is needed for one measurement) so you can perform a repeat determination | |
- | Take at least 500 µL sample for each measurement (200 µL is needed for one measurement) so you can perform a repeat determination | + | |
Freeze biological samples at -80 °C for storage, keep cell-free at 4 °C in the dark | Freeze biological samples at -80 °C for storage, keep cell-free at 4 °C in the dark | ||
To measure the samples thaw at room temperature and fill 200 µL of each sample in one well of a black, flat bottom 96 well microtiter plate (perform at least a repeat determination) | To measure the samples thaw at room temperature and fill 200 µL of each sample in one well of a black, flat bottom 96 well microtiter plate (perform at least a repeat determination) | ||
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Manual gain: 100 | Manual gain: 100 | ||
Integration time: 20 µs | Integration time: 20 µs | ||
+ | </p> | ||
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<br><b><u>subtitle</u></b> | <br><b><u>subtitle</u></b> |
Revision as of 19:49, 25 September 2013
Analytics
subtitle
Fluorescence measurements
Measuring of mRFP with Tecan Infinite® M200 platereader
Take at least 500 µL sample for each measurement (200 µL is needed for one measurement) so you can perform a repeat determination Freeze biological samples at -80 °C for storage, keep cell-free at 4 °C in the dark To measure the samples thaw at room temperature and fill 200 µL of each sample in one well of a black, flat bottom 96 well microtiter plate (perform at least a repeat determination) Measure the fluorescence in a platereader (we used a Tecan Infinite® M200 platereader) with following settings: 20 sec orbital shaking (1 mm amplitude with a frequency of 87.6 rpm) Measurement mode: Top Excitation: 584 nm Emission: 620 nm Number of reads: 25 Manual gain: 100 Integration time: 20 µs
subtitle