Team:Bielefeld-Germany/Labjournal/Cultivation

From 2013.igem.org

(Difference between revisions)

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 Solubilize 20 mg mL-1 Chloramphenicol in 100 % Ethanol
- Store at -20 °C
+Store at -20 °C
 <!--
-<table border=1 style="float:left; padding-left:10px">
-      <col width="150">
-      <col width="50">
-      <col width="50">
-<tr> <th>Reagent</th>	        <th>1x</th>	<th>8x</th> </tr>
-<tr> <td>Hf-Buffer 5x</td>      <td align="right">10.0</td>	<td align="right">88.0</td> </tr>
-<tr> <td>dNTPs (10mM)</td>  	<td align="right">2.0</td>	<td align="right">17.6</td> </tr>
-<tr> <td>Primer1 (10mM)</td>	<td align="right">1.0</td>	<td align="right">8.8</td> </tr>
-<tr> <td>Primer2 (10mM)</td> 	<td align="right">1.0</td>	<td align="right">8.8</td> </tr>
-<tr> <td>Template (16.5ng)</td>	<td align="right">3.0</td>	<td align="right">26.4</td> </tr>
-<tr> <td>DMSO</td>	        <td align="right">4.0</td>    <td align="right">35.2</td> </tr>
-<tr> <td>Phusion (0.5u)</td>        <td align="right">1.0</td>	<td align="right">8.8</td> </tr>
-<tr> <td>H2O</td>    	       <td align="right">26.0</td>	<td align="right">228.8</td> </tr>
-</table>
-<table border=1 style="float:left; margin-left:20px">
-      <col width="50">
-      <col width="50">
-      <col width="50">
-      <col width="50">
-      <col width="50">
-<tr><th colspan="5">Program Phusion</th></tr>
-<tr align="center"><td>98 °C</td><td>98 °C</td><td>68 °C</td><td>72 °C</td><td>72 °C</td></tr>
-<tr align="center"><td>30s</td> <td>10s</td><td>30s</td> <td>1:35</td><td>6:00</td></tr>
-<tr><td></td><td colspan="3" align="center">35 cycles</td><td></td></tr>
-<tr><td colspan="5">
-<b><u>Notes:</u></b>
-<br>Size: 1800bp
-<br>Primer1:
-<br>Primer2:
-<br>
-</td></tr>
-</table>
 -->

Team:Bielefeld-Germany/Labjournal/Cultivation

From 2013.igem.org

Revision as of 15:29, 4 September 2013

Cultivation