Team:Bielefeld-Germany/Labjournal/June

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June

Milesstones





5.Week

Organization

  • iGEM-Team Bielefeld will support the ‘CeBiTec Pupil Academy’ from 26.-30. August with an own experiment.

MFC

Mediators

Cytochromes

Biosafety

Porines









6.Week

Organization

  • Thanks to NEB Biolabs for the free iGEM kit with many useful laboratory things for all German iGEM teams.
  • We are working on our first press release.
  • Having a short radio contribution in the Bielefeld university campus radio (radio 87.9 hertz).


MFC

Mediators

Glycerol dehydrogenase
  • Isolation of shipping vector pSB1C3 out of 2013 Distribution Kit Plate 5 Well 3A with insert Part RFP (J04450) for better transformation characterization (Distribution Kit BioBrick isolation).
  • Transformation of BBa_J04450 into Escherichia coli KRX strain.
  • Plasmid isolation of BBa_J04450.


Cytochromes

Biosafety

Porines












7.Week

Organization

  • Nothing organizational.

MFC

Mediators

Cytochromes

  • Cultivation of Shewanella oneidensis MR-1 in liquid LB medium at 30 °C


  • Isolation of genomic DNA from S. oneidensis
    • 4-2006-451: Conc.:453.1 ng/µl OD260/280: 1.92 OD260/230:2.78
    • 4-2006-452: Conc.:447.1 ng/µl OD260/280: 1.92 OD260/230:2.67
    • Dilution to PCR template: 4-2006-453: 5.5ng/µl


  • Amplification of the mtrCAB cluster
    • Gradient: 55.8-56.7-57.8-59.1-60.4-61.7-62.9-63.9
    • Clear Bands at the expected 5.2kb


  • PCR-CleanUp
    • PCR-cleanup of No.2 and No.5 with Macherey-Nagel-Kit and NanoDrop-Measurement
    • Lane2: 4-2106-451: 7.4 ng/µl
    • Lane5: 4-2106-451: 8.5 ng/µl


Biosafety

Porines











8.Week

Organization

  • Let’s go to Lyon, flights are booked for the European jamboree in Lyon from 11.-13. October 2013.
  • We will participate at ‘BioNRW pHD Student Convention’ in Düsseldorf at 13. July.

MFC

Mediators

Glycerol dehydrogenase
  • Cloning of GldA into pSB1C3 shipping vector with NEB Biobrick assembly Kit did not work as expected.
  • Screening of colonies with colony PCR and Plasmid restriction analysis shows religated pSB1C3 shipping vector.
  • Bands are at size of 2000 bp, the length of linear pSB1C3.
  • Primerdesign for pSB1C3 according an universal usable backbone for Gibson Assembly with Prefix and Suffix specific overlaps:
  • Forward Primer pSB1C3 (23 bp): TACTAGTAGCGGCCGCTGCAGTC
  • Reverse Primer pSB1C3 (23 bp): CTCTAGAAGCGGCCGCGAATTCC


Cytochromes

Biosafety

Porines












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