Team:Bielefeld-Germany/Labjournal/May

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May

Milesstones






1.Week

Organization

  • We were already able to find sponsors for our team: IIT BIEKUBA, IIT Biotech, Evonik, PlasmidFactory and FisherScientific will support us.


MFC

Mediators

  • Glycerol dehydrogenase
    • Primerdesign for isolation of GldA gene from Escherichia coli KRX strain, with overlaps for Biobrick Prefix and Suffix:
    • Forward Primer GldA (43 bp): GAATTCGCGGCCGCTTCTAGATGGACCGCATTATTCAATCACC
    • Reverse Primer GldA (45 bp): CTGCAGCGGCCGCTACTAGTATTATTCCCACTCTTGCAGGAAACG


Cytochromes

Biosafety

Porines









2.Week

Organization

  • Distribution of our team in various subgroups for best work efficiency.
  • We’ve organized a working group barbecue to thank the AG of Dr. Jörn Kalinowski in the CeBiTec for the appropriation of space and support in the laboratory.

MFC

Mediators

Cytochromes

Biosafety

Porines









3.Week

Organization

  • Planning the trip to the European jamboree in Lyon from 11.-13. October 2013.

MFC

Mediators

  • Glycerol dehydrogenase
    • Starting first cultivation of Escherichia coli KRX strain for complete genome isolation.
    • Successful genome isolation of Escherichia coli KRX.
    • Successful PCR on the GldA gene of Escherichia coli KRX strain.
Table 1: Standard Phusion PCR Master Mix.
Table 2: Two step standard Phusion PCR programm for GldA amplification.
    • GldA PCR product was isolated by Agarose gel electrophorese and purificated.
    • Bands are at expected size of 1050 bp.
Figure 1: Agarosegel from PCR on the GldA gene of Escherichia coli KRX strain with forward and reverse primer GldA. For Ladder we used GeneRuler™ 1 kb DNA Ladder fromThermo Scientific.


Cytochromes

Biosafety

Porines









4.Week

Organization

  • We will also take part at the congress "Next generation of biotechnological processes 2020+" in Berlin at 27. June 2013.
  • Having a second presentation at Merck KGaA head office in Darmstadt for explaining our project in detail. We are happy that Merck will be our next supporter in 2013.

MFC

Mediators

Cytochromes

Biosafety

Porines









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