"
Team:CU-Boulder/Notebook/Experiments
From 2013.igem.org
(Difference between revisions)
| (23 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{Template:CU-BoulderHeader}} | {{Template:CU-BoulderHeader}} | ||
| - | < | + | <html> |
| - | + | <div class="shadow-bottom"> | |
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_1_1">Experiment 1.1 - Chemical Transformation Efficiency</a> <a href="https://static.igem.org/mediawiki/2013/0/03/Experiment_1.1_%28ChemicalTransformationEfficiency%29.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>This experiment was designed to test several parameters of our transformation protocol to determine ways of increasing our transformation efficiency.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| - | + | <div class="shadow-bottom"> | |
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_1">Experiment 2.1 - Competent Cell Efficiency</a> <a href="https://static.igem.org/mediawiki/2013/8/80/Experiment_2.1_%28CompetentCellEfficiency%29.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>In this experiment we determined the relative efficiency of three different batches of competent cells; one prepared using a protocol supplied by our advisor, Alex, and the other two prepared according to the iGEM protocol.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| - | < | + | <div class="shadow-bottom"> |
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_2">Experiment 2.2 - Transformation Recovery Period</a> <a href="https://static.igem.org/mediawiki/2013/3/35/Experiment_2.2_%28TransformationRecoveryPeriod%29.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>We tested the effect of varying the recovery period of our transformation protocol on transformation efficiency.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| + | |||
| + | <div class="shadow-bottom"> | ||
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_3">Experiment 2.3 - Transformation Recovery Medium</a> <a href="https://static.igem.org/mediawiki/2013/7/7e/Experiment_2.3_%28TransformationRecoveryMedium%29.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>In this experiment we tried using different types and volumes of growth media during the recovery phase of our transformation protocol to see which worked the best.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| + | |||
| + | <div class="shadow-bottom"> | ||
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_3_1">Experiment 3.1 - Promega Miniprep Protocol</a> <a href="https://static.igem.org/mediawiki/2013/8/80/Experiment_3.1_%28PromegaMiniprepProtocol%29.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>Several parameter of the Promega miniprep protocol were varied in this experiment to investigate their effect on DNA recovery.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| + | |||
| + | <div class="shadow-bottom"> | ||
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_5_1">Experiment 5.1 - Calculating Competent Cell Efficiency</a> <a href="https://static.igem.org/mediawiki/2013/b/b7/Experiment_5.1_%28CalculatingCompetentCellEfficiency%29.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>We calculated the efficiency of our competent cells using iGEM's transformation efficiency kit.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| + | |||
| + | <div class="shadow-bottom"> | ||
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_6_1">Experiment 6.1 - Competent Cell Efficiency</a> <a href="https://static.igem.org/mediawiki/2013/5/52/Experiment_6.1_%28CompetentCellEfficiency%29.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>This experiment was conducted to compare the relative efficiency of a new batch of competent cells to our original supply.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| + | |||
| + | <div class="shadow-bottom"> | ||
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_6_2">Experiment 6.2 - Gel Separation of Proteins Methods </a> <a href="https://static.igem.org/mediawiki/2013/9/98/Experiment_6.2_%28Gel_Separation_of_Proteins_Methods%29.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>In this experiment we tested different ways to separate RFP with an agarose gel.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| + | |||
| + | <div class="shadow-bottom"> | ||
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_10_1">Experiment 10.1 - Preliminary Homemade Miniprep Buffer Experiment</a> <a href="https://static.igem.org/mediawiki/2013/3/3b/Experiment_10.1_%28PreliminaryHomemadeBuffers%29.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>This experiment tests the effectiveness of each of our homemade miniprep buffers individually within the Qiagen miniprep system.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| + | |||
| + | <div class="shadow-bottom"> | ||
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_11_1">Experiment 11.1 - Mini-column Recycling Protocol</a> <a href="https://static.igem.org/mediawiki/2013/8/86/Experiment_11.1_%28Mini-ColumnRecyclingProtocol%29.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>This experiment was conducted to test whether our mini-column recycling protocol was truly effective at removing residual DNA from used miniprep mini-columns.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| + | |||
| + | <div class="shadow-bottom"> | ||
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_11_2">Experiment 11.2 - Homemade vs. Qiagen Miniprep Buffers</a> <a href="https://static.igem.org/mediawiki/2013/a/a2/Experiment_11.2_%28HomemadeVsQiagenBuffers%29.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>Here we tested our homemade miniprep buffers against the commercial Qiagen buffers as a complete set.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| + | |||
| + | <div class="shadow-bottom"> | ||
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_11_3">Experiment 11.3 - Mini-column Recycling Protocol Pt. II</a> <a href="https://static.igem.org/mediawiki/2013/a/a9/Experiment_11.3_%28Mini-ColumnRecyclingProtocol2%29.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>This experiment was designed to compare the effectiveness of recycled mini-columns to new mini-columns.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| + | |||
| + | <div class="shadow-bottom"> | ||
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_12_1">Experiment 12.1 - Calculating Competent Cell Efficiency</a> <a href="https://static.igem.org/mediawiki/2013/d/d4/Experiment_12.1_%28CalculatingCompetentCellEfficiency%29.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>Here we tested the efficiency of our most recent batch of competent cells using iGEM's tranformation efficiency kit.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| + | |||
| + | <div class="shadow-bottom"> | ||
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_14_1">Experiment 14.1 - Gel Separation of Proteins</a> <a href="https://static.igem.org/mediawiki/2013/1/1f/Gel_Separation_of_Proteins.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>In this experiment we tested how effective separating AmilCP from RFP was on an agarose gel.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| + | |||
| + | <div class="shadow-bottom"> | ||
| + | <dl> | ||
| + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/RTXexperiment">Experiment 15.1 - Protein Purification Using RTX</a> <a href="https://static.igem.org/mediawiki/2013/a/a1/RTXExperiment_%281%29.pdf"><i class="icon-download"></i></a></dt> | ||
| + | <dd>This experiment tested the efficacy of RTX as a protein purification method.</dd> | ||
| + | </dl> | ||
| + | </div> | ||
| + | |||
| + | </html> | ||
Latest revision as of 20:32, 25 September 2013
- Experiment 1.1 - Chemical Transformation Efficiency
- This experiment was designed to test several parameters of our transformation protocol to determine ways of increasing our transformation efficiency.
- Experiment 2.1 - Competent Cell Efficiency
- In this experiment we determined the relative efficiency of three different batches of competent cells; one prepared using a protocol supplied by our advisor, Alex, and the other two prepared according to the iGEM protocol.
- Experiment 2.2 - Transformation Recovery Period
- We tested the effect of varying the recovery period of our transformation protocol on transformation efficiency.
- Experiment 2.3 - Transformation Recovery Medium
- In this experiment we tried using different types and volumes of growth media during the recovery phase of our transformation protocol to see which worked the best.
- Experiment 3.1 - Promega Miniprep Protocol
- Several parameter of the Promega miniprep protocol were varied in this experiment to investigate their effect on DNA recovery.
- Experiment 5.1 - Calculating Competent Cell Efficiency
- We calculated the efficiency of our competent cells using iGEM's transformation efficiency kit.
- Experiment 6.1 - Competent Cell Efficiency
- This experiment was conducted to compare the relative efficiency of a new batch of competent cells to our original supply.
- Experiment 6.2 - Gel Separation of Proteins Methods
- In this experiment we tested different ways to separate RFP with an agarose gel.
- Experiment 10.1 - Preliminary Homemade Miniprep Buffer Experiment
- This experiment tests the effectiveness of each of our homemade miniprep buffers individually within the Qiagen miniprep system.
- Experiment 11.1 - Mini-column Recycling Protocol
- This experiment was conducted to test whether our mini-column recycling protocol was truly effective at removing residual DNA from used miniprep mini-columns.
- Experiment 11.2 - Homemade vs. Qiagen Miniprep Buffers
- Here we tested our homemade miniprep buffers against the commercial Qiagen buffers as a complete set.
- Experiment 11.3 - Mini-column Recycling Protocol Pt. II
- This experiment was designed to compare the effectiveness of recycled mini-columns to new mini-columns.
- Experiment 12.1 - Calculating Competent Cell Efficiency
- Here we tested the efficiency of our most recent batch of competent cells using iGEM's tranformation efficiency kit.
- Experiment 14.1 - Gel Separation of Proteins
- In this experiment we tested how effective separating AmilCP from RFP was on an agarose gel.
- Experiment 15.1 - Protein Purification Using RTX
- This experiment tested the efficacy of RTX as a protein purification method.
