Team:CU-Boulder/Notebook/Experiments

From 2013.igem.org

(Difference between revisions)
Line 2: Line 2:
<html>
<html>
 +
<div class="shadow-bottom">
<div class="shadow-bottom">
<dl>
<dl>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_1_1">Experiment 1.1 - Chemical Transformation Efficiency</a> <a href="https://static.igem.org/mediawiki/2013/0/03/Experiment_1.1_%28ChemicalTransformationEfficiency%29.pdf"><i class="icon-download"></i></a></dt>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_1_1">Experiment 1.1 - Chemical Transformation Efficiency</a> <a href="https://static.igem.org/mediawiki/2013/0/03/Experiment_1.1_%28ChemicalTransformationEfficiency%29.pdf"><i class="icon-download"></i></a></dt>
-
<dd>The experiment was designed to test several parameters of our transformation protocol to determine ways of increasing our transformation efficiency.</dd>
+
<dd>This experiment was designed to test several parameters of our transformation protocol to determine ways of increasing our transformation efficiency.</dd>
 +
</dl>
 +
</div>
 +
 
 +
<div class="shadow-bottom">
 +
<dl>
 +
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_1">Experiment 2.1 - Competent Cell Efficiency</a> <a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_1"><i class="icon-download"></i></a></dt>
 +
<dd>In this experiment we determined the relative efficiency of 3 batches of competent cells; one prepared using a protocol supplied by our advisor, Alex, and the other 2 prepared from the iGEM protocol.</dd>
</dl>
</dl>
</div>
</div>

Revision as of 17:19, 15 August 2013

Experiment 1.1 - Chemical Transformation Efficiency
This experiment was designed to test several parameters of our transformation protocol to determine ways of increasing our transformation efficiency.
Experiment 2.1 - Competent Cell Efficiency
In this experiment we determined the relative efficiency of 3 batches of competent cells; one prepared using a protocol supplied by our advisor, Alex, and the other 2 prepared from the iGEM protocol.

1.1 - Chemical Transformation Efficiency

2.1 - Competent Cell Efficiency

2.2 - Transformation Recovery Period

2.3 - Transformation Recovery Medium

3.1 - Promega Miniprep Protocol

5.1 - Calculating Competent Cell Efficiency

6.1 - Competent Cell Efficiency

10.1 - Preliminary Homemade Miniprep Buffers

11.1 - Mini-column Recycling Protocol

11.2 - Homemade Vs. Qiagen Miniprep Buffers

11.3 - Mini-column Recycling Protocol Pt. 2

12.1 - Calculating Competent Cell Efficiency