Team:CU-Boulder/Notebook/Experiments

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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_2">Experiment 2.2 - Transformation Recovery Period</a> <a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_2"><i class="icon-download"></i></a></dt>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_2">Experiment 2.2 - Transformation Recovery Period</a> <a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_2"><i class="icon-download"></i></a></dt>
<dd>We tested the effect of varying the recovery period of our transformation protocol on transformation efficiency.</dd>
<dd>We tested the effect of varying the recovery period of our transformation protocol on transformation efficiency.</dd>
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_3">Experiment 2.3 - Transformation Recovery Medium</a> <a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_3"><i class="icon-download"></i></a></dt>
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<dd>In this experiment we tried using different types and volumes of growth media during the recovery phase of our transformation protocol to see which worked the best.</dd>
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Revision as of 18:06, 15 August 2013

Experiment 1.1 - Chemical Transformation Efficiency
This experiment was designed to test several parameters of our transformation protocol to determine ways of increasing our transformation efficiency.
Experiment 2.1 - Competent Cell Efficiency
In this experiment we determined the relative efficiency of three different batches of competent cells; one prepared using a protocol supplied by our advisor, Alex, and the other two prepared according to the iGEM protocol.
Experiment 2.2 - Transformation Recovery Period
We tested the effect of varying the recovery period of our transformation protocol on transformation efficiency.
Experiment 2.3 - Transformation Recovery Medium
In this experiment we tried using different types and volumes of growth media during the recovery phase of our transformation protocol to see which worked the best.

1.1 - Chemical Transformation Efficiency

2.1 - Competent Cell Efficiency

2.2 - Transformation Recovery Period

2.3 - Transformation Recovery Medium

3.1 - Promega Miniprep Protocol

5.1 - Calculating Competent Cell Efficiency

6.1 - Competent Cell Efficiency

10.1 - Preliminary Homemade Miniprep Buffers

11.1 - Mini-column Recycling Protocol

11.2 - Homemade Vs. Qiagen Miniprep Buffers

11.3 - Mini-column Recycling Protocol Pt. 2

12.1 - Calculating Competent Cell Efficiency