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Team:CU-Boulder/Notebook/Experiments
From 2013.igem.org
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| - | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_1">Experiment 2.1 - Competent Cell Efficiency</a> <a href="https:// | + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_1">Experiment 2.1 - Competent Cell Efficiency</a> <a href="https://static.igem.org/mediawiki/2013/8/80/Experiment_2.1_%28CompetentCellEfficiency%29.pdf"><i class="icon-download"></i></a></dt> |
<dd>In this experiment we determined the relative efficiency of three different batches of competent cells; one prepared using a protocol supplied by our advisor, Alex, and the other two prepared according to the iGEM protocol.</dd> | <dd>In this experiment we determined the relative efficiency of three different batches of competent cells; one prepared using a protocol supplied by our advisor, Alex, and the other two prepared according to the iGEM protocol.</dd> | ||
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| - | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_2">Experiment 2.2 - Transformation Recovery Period</a> <a href="https:// | + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_2">Experiment 2.2 - Transformation Recovery Period</a> <a href="https://static.igem.org/mediawiki/2013/3/35/Experiment_2.2_%28TransformationRecoveryPeriod%29.pdf"><i class="icon-download"></i></a></dt> |
<dd>We tested the effect of varying the recovery period of our transformation protocol on transformation efficiency.</dd> | <dd>We tested the effect of varying the recovery period of our transformation protocol on transformation efficiency.</dd> | ||
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| - | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_3">Experiment 2.3 - Transformation Recovery Medium</a> <a href="https:// | + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_3">Experiment 2.3 - Transformation Recovery Medium</a> <a href="https://static.igem.org/mediawiki/2013/7/7e/Experiment_2.3_%28TransformationRecoveryMedium%29.pdf"><i class="icon-download"></i></a></dt> |
<dd>In this experiment we tried using different types and volumes of growth media during the recovery phase of our transformation protocol to see which worked the best.</dd> | <dd>In this experiment we tried using different types and volumes of growth media during the recovery phase of our transformation protocol to see which worked the best.</dd> | ||
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| - | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_3_1">Experiment 3.1 - Promega Miniprep Protocol</a> <a href="https:// | + | <dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_3_1">Experiment 3.1 - Promega Miniprep Protocol</a> <a href="https://static.igem.org/mediawiki/2013/8/80/Experiment_3.1_%28PromegaMiniprepProtocol%29.pdf"><i class="icon-download"></i></a></dt> |
<dd>Several parameter of the Promega miniprep protocol were varied in this experiment to investigate their effect on DNA recovery.</dd> | <dd>Several parameter of the Promega miniprep protocol were varied in this experiment to investigate their effect on DNA recovery.</dd> | ||
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Revision as of 18:14, 15 August 2013
- Experiment 1.1 - Chemical Transformation Efficiency
- This experiment was designed to test several parameters of our transformation protocol to determine ways of increasing our transformation efficiency.
- Experiment 2.1 - Competent Cell Efficiency
- In this experiment we determined the relative efficiency of three different batches of competent cells; one prepared using a protocol supplied by our advisor, Alex, and the other two prepared according to the iGEM protocol.
- Experiment 2.2 - Transformation Recovery Period
- We tested the effect of varying the recovery period of our transformation protocol on transformation efficiency.
- Experiment 2.3 - Transformation Recovery Medium
- In this experiment we tried using different types and volumes of growth media during the recovery phase of our transformation protocol to see which worked the best.
- Experiment 3.1 - Promega Miniprep Protocol
- Several parameter of the Promega miniprep protocol were varied in this experiment to investigate their effect on DNA recovery.
1.1 - Chemical Transformation Efficiency
2.1 - Competent Cell Efficiency
2.2 - Transformation Recovery Period
2.3 - Transformation Recovery Medium
3.1 - Promega Miniprep Protocol
5.1 - Calculating Competent Cell Efficiency
6.1 - Competent Cell Efficiency
10.1 - Preliminary Homemade Miniprep Buffers
11.1 - Mini-column Recycling Protocol
11.2 - Homemade Vs. Qiagen Miniprep Buffers
