Team:CU-Boulder/Notebook/Experiments

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Experiment 1.1 - Chemical Transformation Efficiency
This experiment was designed to test several parameters of our transformation protocol to determine ways of increasing our transformation efficiency.
Experiment 2.1 - Competent Cell Efficiency
In this experiment we determined the relative efficiency of 3 batches of competent cells; one prepared using a protocol supplied by our advisor, Alex, and the other 2 prepared from the iGEM protocol.

1.1 - Chemical Transformation Efficiency

2.1 - Competent Cell Efficiency

2.2 - Transformation Recovery Period

2.3 - Transformation Recovery Medium

3.1 - Promega Miniprep Protocol

5.1 - Calculating Competent Cell Efficiency

6.1 - Competent Cell Efficiency

10.1 - Preliminary Homemade Miniprep Buffers

11.1 - Mini-column Recycling Protocol

11.2 - Homemade Vs. Qiagen Miniprep Buffers

11.3 - Mini-column Recycling Protocol Pt. 2

12.1 - Calculating Competent Cell Efficiency