Team:CU-Boulder/Notebook/Protocols

From 2013.igem.org

(Difference between revisions)
Line 5: Line 5:
<dl>
<dl>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/EmbeddingPDF">Embedding PDF Files</a> <a href="https://static.igem.org/mediawiki/2013/9/99/Embedding_a_PDF_file_on_the_iGEM_Team_Websites.pdf"><i class="icon-download"></i></a></dt>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/EmbeddingPDF">Embedding PDF Files</a> <a href="https://static.igem.org/mediawiki/2013/9/99/Embedding_a_PDF_file_on_the_iGEM_Team_Websites.pdf"><i class="icon-download"></i></a></dt>
-
<p></p>
 
<dd>In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.</dd>
<dd>In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.</dd>
</dl>
</dl>
Line 13: Line 12:
<dl>
<dl>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/GoogleDocEmbed">Embedding Google Docs</a>  <a href="https://static.igem.org/mediawiki/2013/0/04/Embedding_Google_Docs_on_the_Wiki_-_Google_Drive.pdf"><i class="icon-download"></i></a></dt>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/GoogleDocEmbed">Embedding Google Docs</a>  <a href="https://static.igem.org/mediawiki/2013/0/04/Embedding_Google_Docs_on_the_Wiki_-_Google_Drive.pdf"><i class="icon-download"></i></a></dt>
-
<p></p>
 
<dd>Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.</dd>
<dd>Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.</dd>
</dl>
</dl>
Line 21: Line 19:
<dl>
<dl>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ChemicallyCompetentCells">Chemically Competent Cells</a> <a href="https://static.igem.org/mediawiki/2013/d/da/Chemically_Competent_Cells.pdf"><i class="icon-download"></i></a></dt>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ChemicallyCompetentCells">Chemically Competent Cells</a> <a href="https://static.igem.org/mediawiki/2013/d/da/Chemically_Competent_Cells.pdf"><i class="icon-download"></i></a></dt>
-
<p></p>
 
<dd>This protocol details how to prepare chemically competent cells.</dd>
<dd>This protocol details how to prepare chemically competent cells.</dd>
</dl>
</dl>
Line 29: Line 26:
<dl>
<dl>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ChemicalTransformations">Chemical Transformations</a> <a href="https://static.igem.org/mediawiki/2013/1/15/Chemical_Transformations.pdf"><i class="icon-download"></i></a></dt>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ChemicalTransformations">Chemical Transformations</a> <a href="https://static.igem.org/mediawiki/2013/1/15/Chemical_Transformations.pdf"><i class="icon-download"></i></a></dt>
-
<p></p>
 
<dd>In this protocol we cover how to use our chemically competent cells to perform transformations.</dd>
<dd>In this protocol we cover how to use our chemically competent cells to perform transformations.</dd>
</dl>
</dl>
Line 37: Line 33:
<dl>
<dl>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/PlasmidMinipreps">Plasmid Minipreps</a> <a href="https://static.igem.org/mediawiki/2013/0/08/Plasmid_Miniprep.pdf"><i class="icon-download"></i></a></dt>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/PlasmidMinipreps">Plasmid Minipreps</a> <a href="https://static.igem.org/mediawiki/2013/0/08/Plasmid_Miniprep.pdf"><i class="icon-download"></i></a></dt>
-
<p></p>
 
<dd>Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.</dd>
<dd>Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.</dd>
</dl>
</dl>
Line 45: Line 40:
<dl>
<dl>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/RestrictionDigests">Restriction (Double) Digests</a> <a href="https://static.igem.org/mediawiki/2013/f/f7/Restriction_Digests.pdf"><i class="icon-download"></i></a></dt>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/RestrictionDigests">Restriction (Double) Digests</a> <a href="https://static.igem.org/mediawiki/2013/f/f7/Restriction_Digests.pdf"><i class="icon-download"></i></a></dt>
-
<p></p>
 
<dd>Our method of performing restriction double digests is detailed in this protocol.</dd>
<dd>Our method of performing restriction double digests is detailed in this protocol.</dd>
</dl>
</dl>
Line 53: Line 47:
<dl>
<dl>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/HomemadeBufferCompositions">Homemade Buffer Compositions</a> <a href="https://static.igem.org/mediawiki/2013/8/85/Homemade_Buffer_Compositions.pdf"><i class="icon-download"></i></a></dt>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/HomemadeBufferCompositions">Homemade Buffer Compositions</a> <a href="https://static.igem.org/mediawiki/2013/8/85/Homemade_Buffer_Compositions.pdf"><i class="icon-download"></i></a></dt>
-
<p></p>
 
<dd>The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.</dd>
<dd>The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.</dd>
</dl>
</dl>
Line 61: Line 54:
<dl>
<dl>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/RecyclingMinicolumns">Recycling DNA Mini-columns</a> <a href="https://static.igem.org/mediawiki/2013/d/dc/Recycling_DNA_Mini-columns.pdf"><i class="icon-download"></i></a></dt>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/RecyclingMinicolumns">Recycling DNA Mini-columns</a> <a href="https://static.igem.org/mediawiki/2013/d/dc/Recycling_DNA_Mini-columns.pdf"><i class="icon-download"></i></a></dt>
-
<p></p>
 
<dd>This protocol covers how to recycle mini-columns used in plasmid miniprep systems.</dd>
<dd>This protocol covers how to recycle mini-columns used in plasmid miniprep systems.</dd>
</dl>
</dl>

Revision as of 16:48, 22 August 2013

Embedding PDF Files
In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.
Embedding Google Docs
Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.
Chemically Competent Cells
This protocol details how to prepare chemically competent cells.
Chemical Transformations
In this protocol we cover how to use our chemically competent cells to perform transformations.
Plasmid Minipreps
Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.
Restriction (Double) Digests
Our method of performing restriction double digests is detailed in this protocol.
Homemade Buffer Compositions
The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.
Recycling DNA Mini-columns
This protocol covers how to recycle mini-columns used in plasmid miniprep systems.