Team:CU-Boulder/Notebook/Protocols

From 2013.igem.org

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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ChemicallyCompetentCells">Chemically Competent Cells</a> <a href="https://static.igem.org/mediawiki/2013/d/da/Chemically_Competent_Cells.pdf"><i class="icon-download"></i></a></dt>
<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ChemicallyCompetentCells">Chemically Competent Cells</a> <a href="https://static.igem.org/mediawiki/2013/d/da/Chemically_Competent_Cells.pdf"><i class="icon-download"></i></a></dt>
<dd>This protocol details how to prepare chemically competent cells.</dd>
<dd>This protocol details how to prepare chemically competent cells.</dd>
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<dt>Experiments</dt>
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<dd><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_1">Experiment 2.1 - Competent Cell Efficiency</a></dt>
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Revision as of 16:51, 22 August 2013

Embedding PDF Files
In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.
Embedding Google Docs
Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.
Chemically Competent Cells
This protocol details how to prepare chemically competent cells.

Experiments
Experiment 2.1 - Competent Cell Efficiency
Chemical Transformations
In this protocol we cover how to use our chemically competent cells to perform transformations.
Plasmid Minipreps
Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.
Restriction (Double) Digests
Our method of performing restriction double digests is detailed in this protocol.
Homemade Buffer Compositions
The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.
Recycling DNA Mini-columns
This protocol covers how to recycle mini-columns used in plasmid miniprep systems.