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Team:CU-Boulder/Notebook/Protocols
From 2013.igem.org
(Difference between revisions)
| Line 66: | Line 66: | ||
<dd>The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.</dd> | <dd>The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.</dd> | ||
</dl> | </dl> | ||
| + | <h4>Related Experiments</h4> | ||
| + | <ul> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_10_1">Experiment 10.1 - Preliminary Homemade Miniprep Buffer Experiment</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_11_2">Experiment 11.2 - Homemade vs. Qiagen Miniprep Buffers</a></li> | ||
| + | </ul> | ||
</div> | </div> | ||
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<dd>This protocol covers how to recycle mini-columns used in plasmid miniprep systems.</dd> | <dd>This protocol covers how to recycle mini-columns used in plasmid miniprep systems.</dd> | ||
</dl> | </dl> | ||
| + | <h4>Related Experiments</h4> | ||
| + | <ul> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_11_1">Experiment 11.1 - Mini-column Recycling Protocol</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_11_3">Experiment 11.3 - Mini-column Recycling Protocol Pt. II</a></li> | ||
</div> | </div> | ||
Revision as of 17:18, 22 August 2013
- Embedding PDF Files
- In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.
- Embedding Google Docs
- Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.
- Chemically Competent Cells
- This protocol details how to prepare chemically competent cells.
Related Experiments
- Chemical Transformations
- In this protocol we cover how to use our chemically competent cells to perform transformations.
Related Experiments
- Plasmid Minipreps
- Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.
Related Experiments
- Restriction (Double) Digests
- Our method of performing restriction double digests is detailed in this protocol.
- Homemade Buffer Compositions
- The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.
Related Experiments
- Recycling DNA Mini-columns
- This protocol covers how to recycle mini-columns used in plasmid miniprep systems.
