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Team:CU-Boulder/Project
From 2013.igem.org
- Project Abstract
- Restriction enzymes are invaluable tools in the field synthetic biology, and are essential for carrying out BioBrick assembly. However, these enzymes also represent a significant portion of the cost associated with gene cloning and DNA analysis. Here at the University of Colorado-Boulder, our vision is to develop the parts and methods necessary to produce and purify these enzymes cheaply and reliably. In order to make our project successful, we will be creating the BioBrick constructs that contain the restriction enzymes we are trying to produce. In addition, we will be developing procedures that allow us to purify these enzymes cheaply and effectively. The end result of our project will be the completion of several RE prep kits which will contain all of the components necessary to create working stocks of several commonly used restriction enzymes, including EcoRI, XbaI, and PstI which are all part of the BioBrick standard.
