Team:Calgary/Notebook/Protocols/VerificationDigest

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Verification Digest

Reagents and Materials

  • Restriction enzymes
  • Appropriate buffer
  • Plasmid purified DNA
  • 1% agarose gel

Protocol

  1. In a tube, add (per reaction):
    • 4µL Plasmid purified DNA
    • 1µL appropriate buffer
    • 0.2µL enzyme 1
    • 0.2µL enzyme 2
    • 3.6µL ddH2O (deionized water)
  2. Incubate at 37 degrees Celsius for 1h
  3. Run 10µL in a 1% agarose gel at 100V