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Team:Calgary/Project/Achievements
From 2013.igem.org
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<ul><li>Amended two TALEs from the registry and resubmitted these parts. We optimized the TALEs such that they can be expressed in <i> E. coli </i> by removing the eukaryotic kozak sequence present in the original parts, codon optimizing it for <i> E. coli</i> and added a his-6 tag such that this protein can be purified using affinity chromatography.</li> | <ul><li>Amended two TALEs from the registry and resubmitted these parts. We optimized the TALEs such that they can be expressed in <i> E. coli </i> by removing the eukaryotic kozak sequence present in the original parts, codon optimizing it for <i> E. coli</i> and added a his-6 tag such that this protein can be purified using affinity chromatography.</li> | ||
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| + | <li> We submitted two novel reporters: beta-lactamase and ferritin. We showed that these enzymes </li> | ||
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| + | <li> We characterized beta-lactamase as a colorimetric reporter using phenol red. We also showed that beta | ||
<li> | <li> | ||
Revision as of 01:59, 28 September 2013
Achievements
Achievements
For our Sensorwe...
- Amended two TALEs from the registry and resubmitted these parts. We optimized the TALEs such that they can be expressed in E. coli by removing the eukaryotic kozak sequence present in the original parts, codon optimizing it for E. coli and added a his-6 tag such that this protein can be purified using affinity chromatography.
- We submitted two novel reporters: beta-lactamase and ferritin. We showed that these enzymes
- We characterized beta-lactamase as a colorimetric reporter using phenol red. We also showed that beta
