Human Practices

Our goal is to lessen enterohemorrhagic E. coli in beef products, and to deal with this industrial problem, we worked with key "steakholders" at key points of the production process. These conversations shaped our entire project and are the foundation of our human practices. Our methodology toward human practices was to tailor our detection system to the end users of the system. We made the following achievements in our human practices:

• Informed design: Input from four key industry stakeholders was used to redefine our project. Through these conversations, we assessed management of E. coli in the current industry and refocused our energy to build a novel tool for management of this pathogen prior to slaughter. We used input from these stakeholders to ensure that our system is easy to use, cost effective, and ultimately feasible for its application.

• Biological and user safety: Our final prototype is comprised of in vitro protein components from recombinant bacteria. This design alleviates concerns over escape of synthetic organisms into the outside environment. We also thought about the sensitivity requirements for differentiating E. coli shedding in beef cattle, particularly with regard to false positives and false negatives.

Informed Design

Safety