Team:Calgary/Sandbox/Notebook/Protocols/RehydrationOfRegistryDNA

From 2013.igem.org

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<h1>Rehydration of Registry DNA</h1>
<h1>Rehydration of Registry DNA</h1>
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<p>Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used.</p>
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<h2>Reagents and Reagents</h2>
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<ul>
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<li>Biobrick parts</li>
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<li>ddH<sub>2</sub>O (deionized water)</li>
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</ul>
<h2>Protocol</h2>
<h2>Protocol</h2>
<ol>
<ol>
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<li>Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used</li>
 
<li>Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li>
<li>Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li>
<li>Add 10 μL of ddH<sub>2</sub>O (deionized water)</li>
<li>Add 10 μL of ddH<sub>2</sub>O (deionized water)</li>

Latest revision as of 01:18, 18 September 2013

Rehydration of Registry DNA

Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used.

Reagents and Reagents

  • Biobrick parts
  • ddH2O (deionized water)

Protocol

  1. Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want
  2. Add 10 μL of ddH2O (deionized water)
  3. Let the water incubate at room temperature for 10 minutes
  4. Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies

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