Team:Calgary/Sandbox/Notebook/Protocols/RehydrationOfRegistryDNA

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Rehydration of Registry DNA

Protocol

  1. Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used
  2. Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want
  3. Add 10 μL of ddH2O (deionized water)
  4. Let the water incubate at room temperature for 10 minutes
  5. Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies