Team:Evry/ChemicalTools

From 2013.igem.org

Revision as of 00:58, 3 October 2013 by Batou (Talk | contribs)

Iron coli project

Chemical reasoning

There are 6 enzyms involved in the natural process of the enterobactin production:

  • EntA :
  • EntB :
  • EntC :
  • EntD :
  • EntE :
  • EntF :
The production of each one of those enzymes can thus become a rate-limiting-step, when it comes to mass enterobactin production.


For now, we consider each one of these steps as a simple chemical reaction:

We are using the enzymatic kinetic model of Michaelis-Menten, which divides each reaction into two consecutives steps:

The speed of the reaction is calculated as below:

The steady state approximation gives us:

And thus,

where and are classic kinetic parameters.

Important result:

For simple enzymatic reactions (one reagent, one product and one enzyme) with the steady state approximation, we can directly determine the speed: