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Team:Evry/Notebook/testbapt
From 2013.igem.org
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<tr> | <tr> | ||
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| - | + | Natural Promotor | |
</th> | </th> | ||
<th align="center"> | <th align="center"> | ||
| - | + | Miniprep | |
</th> | </th> | ||
<th align="center"> | <th align="center"> | ||
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</th> | </th> | ||
<th align="center"> | <th align="center"> | ||
| - | + | Date | |
</th> | </th> | ||
<th align="center"> | <th align="center"> | ||
| - | + | Biobricks | |
</th> | </th> | ||
</tr> | </tr> | ||
Revision as of 10:03, 20 August 2013
| Natural Promotor | Miniprep | Concentration | Date | Biobricks |
|---|---|---|---|---|
|
Fur Binding Site n°1
|
1 | 50.7 ng/µL | 1.62 | 0.68 |
| 2 | 21.1 ng/µL | 1.69 | 0.44 | |
| 3 | 21.6 ng/µL | 1.20 | 0.13 | |
| 4 | 70.8 ng/µL | 1.66 | 0.72 | |
|
Fur Binding Site n°2
|
1 | 39.1 ng/µL | 1.23 | 0.40 |
| 2 | 25.1 ng/µL | 1.37 | 0.29 | |
| 3 | 61.1 ng/µL | 1.57 | 0.71 | |
| 4 | 54.7 ng/µL | 1.62 | 0.68 | |
|
Fur Binding Site n°3
|
2 | 38.4 ng/µL | 1.84 | 0.78 |
| 3 | 114.6 ng/µL | 1.66 | 0.78 | |
| 4 | 39.2 ng/µL | 1.87 | 1.21 | |
|
Fur Binding Site n°5
|
1 | 77.7 ng/µL | 1.76 | 0.99 |
| 3 | 78.4 ng/µL | 1.61 | 0.81 | |
| 4 | 72.7 ng/µL | 1.85 | 4.23 | |
|
Fur Binding Site n°6
|
1 | 70.4 ng/µL | 1.76 | 2.16 |
| 2 | 91.5 ng/µL | 1.55 | 1.21 | |
| 3 | 65.4 ng/µL | 1.70 | 1.52 | |
| 4 | 85.6 ng/µL | 1.72 | 1.65 | |
|
Fur Binding Site n°7
|
1 | 53.5 ng/µL | 1.76 | 1.93 |
| 2 | 77.6 ng/µL | 1.69 | 1.22 | |
| 3 | 72.3 ng/µL | 1.68 | 1.22 | |
|
Fur Binding Site n°8
|
1 | 73.9 ng/µL | 1.58 | 0.84 |
| 2 | 51.3 ng/µL | 1.75 | 1.10 | |
| 3 | 45.9 ng/µL | 1.85 | 1.43 | |
| 4 | 46.1 ng/µL | 1.88 | 1.61 | |
|
Fur Binding Site n°9
|
1 | 108.3 ng/µL | 1.78 | 1.37 |
| 2 | 67.0 ng/µL | 1.88 | 1.61 | |
| 3 | 103.5 ng/µL | 1.87 | 1.79 | |
| 4 | 28.2 ng/µL | 2.06 | 1.76 | |
|
Fur Binding Site n°10
|
1 | 29.5 ng/µL | 1.95 | 1.22 |
| 2 | 70.0 ng/µL | 1.90 | 1.67 | |
| 3 | 46.0 ng/µL | 1.89 | 1.62 | |
| 4 | 55.8 ng/µL | 1.88 | 1.44 | |
|
Fur Binding Site n°11
|
1 | 55.3 ng/µL | 1.85 | 1.49 |
| 2 | 87.1 ng/µL | 1.81 | 1.37 | |
| 3 | 57.7 ng/µL | 1.84 | 1.44 | |
| 4 | 21.7 ng/µL | 2.06 | 1.20 | |
|
Fur Binding Site n°15
|
1 | 16.2 ng/µL | 2.13 | 1.20 |
| 3 | 47.4 ng/µL | 1.78 | 1.16 | |
| 4 | 63.2 ng/µL | 1.78 | 1.16 |
Note: Fur Binding Sites n°4, 12, 13 and 14 are missing.
3rd construction
Sequencing preparation
Preculture with LB medium (with carbenicillin) have been launched for Top10 transformed with our plasmid which contains the sfGFP under the control of Lac O promoter. After one night of culture minipreps of 3 different clones have been realized using the miniprep kit from Machrey Nagel.
| Clone | Concentration | 260/280 | 260/230 |
|---|---|---|---|
| 1 | 25.7 ng/µL | 2.02 | 2.29 |
| 2 | 27.0 ng/µL | 2.09 | 1.78 |
| 3 | 79.7 ng/µL | 1.86 | 1.60 |
