Team:Evry/Notebook/testbapt

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Iron coli project

Natural Promotor Miniprep Concentration (ng/µL) Date Biobricks

Ace B

AceB 1 Lac I 66,4 14/08 No
AceB 2 Lac I 53,8 14/08 No
Ace 4 Lac I 64,9 14/08 No
Ace B 1 sfGFP 166,1 05/08 No
Ace B 2 sfGFP 192,0 05/08 No

Ent C

Fec A

Fec A 1 sfGFP 05/08 No

Fur Binding Site n°5
(with sfGFP)

 1 77.7 ng/µL 1.76 0.99
3 78.4 ng/µL 1.61 0.81
4 72.7 ng/µL 1.85 4.23

Fur Binding Site n°6
(with sfGFP)

 1 70.4 ng/µL 1.76 2.16
2 91.5 ng/µL 1.55 1.21
3 65.4 ng/µL 1.70 1.52
4 85.6 ng/µL 1.72 1.65

Fur Binding Site n°7
(with sfGFP)

 1 53.5 ng/µL 1.76 1.93
2 77.6 ng/µL 1.69 1.22
3 72.3 ng/µL 1.68 1.22

Fur Binding Site n°8
(with sfGFP)

 1 73.9 ng/µL 1.58 0.84
2 51.3 ng/µL 1.75 1.10
3 45.9 ng/µL 1.85 1.43
4 46.1 ng/µL 1.88 1.61

Fur Binding Site n°9
(with sfGFP)

 1 108.3 ng/µL 1.78 1.37
2 67.0 ng/µL 1.88 1.61
3 103.5 ng/µL 1.87 1.79
4 28.2 ng/µL 2.06 1.76

Fur Binding Site n°10
(with sfGFP)

 1 29.5 ng/µL 1.95 1.22
2 70.0 ng/µL 1.90 1.67
3 46.0 ng/µL 1.89 1.62
4 55.8 ng/µL 1.88 1.44

Fur Binding Site n°11
(with sfGFP)

 1 55.3 ng/µL 1.85 1.49
2 87.1 ng/µL 1.81 1.37
3 57.7 ng/µL 1.84 1.44
4 21.7 ng/µL 2.06 1.20

Fur Binding Site n°15
(with sfGFP)

 1 16.2 ng/µL 2.13 1.20
3 47.4 ng/µL 1.78 1.16
4 63.2 ng/µL 1.78 1.16

Note: Fur Binding Sites n°4, 12, 13 and 14 are missing.

3rd construction

Sequencing preparation

Preculture with LB medium (with carbenicillin) have been launched for Top10 transformed with our plasmid which contains the sfGFP under the control of Lac O promoter. After one night of culture minipreps of 3 different clones have been realized using the miniprep kit from Machrey Nagel.

Clone Concentration 260/280 260/230
1 25.7 ng/µL 2.02 2.29
2 27.0 ng/µL 2.09 1.78
3 79.7 ng/µL 1.86 1.60

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