Protocol for transformation

Principle

Preparation

For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA. IF the ligation protocol is a golden gate, add 5 µL instead.
Let 30 minutes on ice.
Let the cells at 42°C for exactly 50 seconds.
Let 5 minutes on ice.
Resuspend the cells with 1 mL of LB spread on a petri dish then let it at 37°C.

Preparation