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Team:Evry/Protocols/02
From 2013.igem.org
Transformation
In this step, bacteria that were prepared to be in state of competence can integrate the DNA (plasmid) from the environment. The difference of temperatureGoal
Preparation
For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA. If the ligation protocol is a
Golden Gate , add 5 µL instead.
Let 30 minutes on ice.
Let the cells at 42°C for exactly 50 seconds.
Let 5 minutes on ice.
Resuspend the cells with 1 mL of LB spread on a petri dish then let it at 37°C.
Tests
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