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Team:Evry/Protocols/07
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<h2> Principle</h2> | <h2> Principle</h2> | ||
| - | <img src='http://upload.wikimedia.org/wikipedia/commons/8/87/PCR.svg' alt=' | + | <img src='http://upload.wikimedia.org/wikipedia/commons/8/87/PCR.svg' alt='PCR' align='right' style="margin-right:20px;" height='500px'/> |
<p>Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence. <br> | <p>Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence. <br> | ||
PCR is divided into 5 steps:<br> | PCR is divided into 5 steps:<br> | ||
Revision as of 11:45, 26 August 2013
PCR
Principle
Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence.
PCR is divided into 5 steps:
-First denaturation
-Denaturation step
-Anealing step
-Elongation step
-Final step
The 2nd, 3th and 4th steps are repeated 20-40 cycles.
Preparation
Optimisation
Number of primer
Temperature
Gel electrophoresis analysis
Principle
Preparation
Analysis
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