PCR and gel electrophoresis analysis

PCR

Principle

Polymerase Chain Reaction is a molecular biology method used to amplify a small amount of genetic material (DNA or RNA), using specific primers of a target sequence.
PCR is divided into 5 steps:
First denaturation
Denaturation step
Anealing step
Elongation step
Final step
The 2nd, 3th and 4th steps are repeated 20-40 cycles.

Preparation

Optimisation

Number of primer

Temperature

Gel electrophoresis analysis

Principle

Preparation

Analysis