Team:OUC-China

From 2013.igem.org

(Difference between revisions)
Line 5: Line 5:
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
<link href="http://netdna.bootstrapcdn.com/bootstrap/3.0.0/css/bootstrap.min.css" rel="stylesheet" media="screen">
<link href="http://netdna.bootstrapcdn.com/bootstrap/3.0.0/css/bootstrap.min.css" rel="stylesheet" media="screen">
 +
<script src="http://code.jquery.com/jquery.min.js"></script>
<style>
<style>
 +
/*the igem Global setting*/
 +
* {
 +
  margin: 0;
 +
  padding: 0;
 +
  -webkit-font-smoothing: antialiased;
 +
  -webkit-box-sizing: border-box;
 +
  -moz-box-sizing: border-box;
 +
  box-sizing: border-box;
 +
}
#globalWrapper {
#globalWrapper {
font-size: 100%;
font-size: 100%;
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background-color: #FFFFFF;
background-color: #FFFFFF;
}
}
-
    /* Carousel base class */
+
/*the nav settings
-
    .carousel {
+
-
      margin-bottom: 60px;
+
-
    }
+
-
 
+
-
    .carousel .container {
+
-
      position: relative;
+
-
      z-index: 9;
+
-
    }
+
-
 
+
-
    .carousel-control {
+
-
      height: 80px;
+
-
      margin-top: 0;
+
-
      font-size: 120px;
+
-
      text-shadow: 0 1px 1px rgba(0,0,0,.4);
+
-
      background-color: transparent;
+
-
      border: 0;
+
-
      z-index: 10;
+
-
    }
+
-
 
+
-
    .carousel .item {
+
-
      height: 400px;
+
-
    }
+
-
    .carousel img {
+
-
      position: absolute;
+
-
      top: 0;
+
-
      left: 0;
+
-
      min-width: 100%;
+
-
      height: 400px;
+
-
    }
+
-
 
+
-
    .carousel-caption {
+
-
      background-color: transparent;
+
-
      position: static;
+
-
      max-width: 550px;
+
-
      padding: 0 20px;
+
-
      margin-top: 200px;
+
-
    }
+
-
    .carousel-caption h1,
+
-
    .carousel-caption .lead {
+
-
      margin: 0;
+
-
      line-height: 1.25;
+
-
      color: #fff;
+
-
      text-shadow: 0 1px 1px rgba(0,0,0,.4);
+
-
    }
+
-
    .carousel-caption .btn {
+
-
      margin-top: 10px;
+
-
    }
+
-
    /* MARKETING CONTENT
+
-
    -------------------------------------------------- */
+
-
 
+
-
    /* Center align the text within the three columns below the carousel */
+
-
    .marketing .span6 {
+
-
      text-align: center;
+
-
    }
+
-
    .marketing h2 {
+
-
      font-weight: normal;
+
-
      margin-left: 200px;
+
-
      margin-right: 60px;
+
-
    }
+
-
    .marketing .span6 p {
+
-
      margin-left: 200px;
+
-
      margin-right: 60px;
+
-
    }
+
-
    /* Featurettes
+
-
    ------------------------- */
+
-
 
+
-
    .featurette-divider {
+
-
      margin: 80px 0; /* Space out the Bootstrap <hr> more */
+
-
    }
+
-
    .featurette {
+
-
      padding-top: 120px; /* Vertically center images part 1: add padding above and below text. */
+
-
      overflow: hidden; /* Vertically center images part 2: clear their floats. */
+
-
    }
+
-
    .featurette-image {
+
-
      margin-top: -120px; /* Vertically center images part 3: negative margin up the image the same amount of the padding to center it. */
+
-
    }
+
-
 
+
-
    /* Give some space on the sides of the floated elements so text doesn't run right into it. */
+
-
    .featurette-image.pull-left {
+
-
      margin-right: 40px;
+
-
    }
+
-
    .featurette-image.pull-right {
+
-
      margin-left: 40px;
+
-
    }
+
-
 
+
-
    /* Thin out the marketing headings */
+
-
    .featurette-heading {
+
-
      font-size: 50px;
+
-
      font-weight: 300;
+
-
      line-height: 1;
+
-
      letter-spacing: -1px;
+
-
    }
+
-
 
+
-
    /* RESPONSIVE CSS
+
-
    -------------------------------------------------- */
+
-
 
+
-
    @media (max-width: 979px) {
+
-
 
+
-
      .container.navbar-wrapper {
+
-
        margin-bottom: 0;
+
-
        width: auto;
+
-
      }
+
-
      .navbar-inner {
+
-
        border-radius: 0;
+
-
        margin: -20px 0;
+
-
      }
+
-
 
+
-
      .carousel .item {
+
-
        height: 400px;
+
-
      }
+
-
      .carousel img {
+
-
        width: auto;
+
-
        height: 400px;
+
-
      }
+
-
 
+
-
      .featurette {
+
-
        height: auto;
+
-
        padding: 0;
+
-
      }
+
-
      .featurette-image.pull-left,
+
-
      .featurette-image.pull-right {
+
-
        display: block;
+
-
        float: none;
+
-
        max-width: 40%;
+
-
        margin: 0 auto 20px;
+
-
      }
+
-
    }
+
-
 
+
-
 
+
-
    @media (max-width: 767px) {
+
-
 
+
-
      .navbar-inner {
+
-
        margin: -20px;
+
-
      }
+
-
 
+
-
      .carousel {
+
-
        margin-left: -20px;
+
-
        margin-right: -20px;
+
-
      }
+
-
      .carousel .container {
+
-
 
+
-
      }
+
-
      .carousel .item {
+
-
        height: 400px;
+
-
      }
+
-
      .carousel img {
+
-
        height: 400px;
+
-
      }
+
-
      .carousel-caption {
+
-
        width: 65%;
+
-
        padding: 0 70px;
+
-
        margin-top: 100px;
+
-
      }
+
-
      .carousel-caption h1 {
+
-
        font-size: 30px;
+
-
      }
+
-
      .carousel-caption .lead,
+
-
      .carousel-caption .btn {
+
-
        font-size: 18px;
+
-
      }
+
-
 
+
-
      .marketing .span4 + .span4 {
+
-
        margin-top: 40px;
+
-
      }
+
-
 
+
-
      .featurette-heading {
+
-
        font-size: 30px;
+
-
      }
+
-
      .featurette .lead {
+
-
        font-size: 18px;
+
-
        line-height: 1.5;
+
-
      }
+
-
 
+
-
    }
+
-
+
-
/*the nav settings
+
----------------------------------------------- */
----------------------------------------------- */
.navbar-inverse .navbar-nav>.open>a, .navbar-inverse .navbar-nav>.open>a:hover, .navbar-inverse .navbar-nav>.open>a:focus{
.navbar-inverse .navbar-nav>.open>a, .navbar-inverse .navbar-nav>.open>a:hover, .navbar-inverse .navbar-nav>.open>a:focus{
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   padding:15px 30px;
   padding:15px 30px;
   margin:0;
   margin:0;
 +
}
 +
.navbar-inverse .navbar-brand:hover{
 +
color: #1abc9c;
}
}
.navbar-inverse{
.navbar-inverse{
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.dropdown-submenu:hover>a:after{border-left-color:#ffffff;}
.dropdown-submenu:hover>a:after{border-left-color:#ffffff;}
.dropdown-submenu.pull-left{float:none;}.dropdown-submenu.pull-left>.dropdown-menu{left:-100%;margin-left:10px;-webkit-border-radius:6px 0 6px 6px;-moz-border-radius:6px 0 6px 6px;border-radius:6px 0 6px 6px;}
.dropdown-submenu.pull-left{float:none;}.dropdown-submenu.pull-left>.dropdown-menu{left:-100%;margin-left:10px;-webkit-border-radius:6px 0 6px 6px;-moz-border-radius:6px 0 6px 6px;border-radius:6px 0 6px 6px;}
 +
 +
/*the unslider css*/
 +
.banner {
 +
  position: relative;
 +
  width: 100%;
 +
  overflow: auto;
 +
 +
  font-size: 18px;
 +
  line-height: 24px;
 +
  text-align: center;
 +
 +
  color: rgba(255,255,255,.6);
 +
  text-shadow: 0 0 1px rgba(0,0,0,.05), 0 1px 2px rgba(0,0,0,.3);
 +
 +
  background: #1abc9c;
 +
  box-shadow: 0 1px 2px rgba(0,0,0,.25);
 +
}
 +
  .banner ul {
 +
    list-style: none;
 +
    width: 300%;
 +
  }
 +
  .banner ul li {
 +
    display: block;
 +
    float: left;
 +
    width: 33%;
 +
    min-height: 350px;
 +
 +
    -o-background-size: 100% 100%;
 +
    -ms-background-size: 100% 100%;
 +
    -moz-background-size: 100% 100%;
 +
    -webkit-background-size: 100% 100%;
 +
    background-size: 100% 100%;
 +
 +
    box-shadow: inset 0 -3px 6px rgba(0,0,0,.1);
 +
  }
 +
 +
  .banner .inner {
 +
    padding: 160px 0 110px;
 +
  }
 +
 +
  .banner h1, .banner h2 {
 +
    font-size: 40px;
 +
    line-height: 52px;
 +
 +
    color: #fff;
 +
  }
 +
 +
  .banner .btn {
 +
    display: inline-block;
 +
    margin: 25px 0 0;
 +
    padding: 9px 22px 7px;
 +
    clear: both;
 +
 +
    color: #fff;
 +
    font-size: 12px;
 +
    font-weight: bold;
 +
    text-transform: uppercase;
 +
    text-decoration: none;
 +
 +
    border: 2px solid rgba(255,255,255,.4);
 +
    border-radius: 5px;
 +
  }
 +
    .banner .btn:hover {
 +
      background: rgba(255,255,255,.05);
 +
    }
 +
    .banner .btn:active {
 +
      -webkit-filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
 +
      -moz-filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
 +
      -ms-filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
 +
      -o-filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
 +
      filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
 +
    }
 +
 +
  .banner .btn, .banner .dot {
 +
    -webkit-filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
 +
    -moz-filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
 +
    -ms-filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
 +
    -o-filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
 +
    filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
 +
  }
 +
 +
  .banner .dots {
 +
    position: absolute;
 +
    left: 0;
 +
    right: 0;
 +
    bottom: 20px;
 +
  }
 +
    .banner .dots li {
 +
      display: inline-block;
 +
      width: 10px;
 +
      height: 10px;
 +
      margin: 0 4px;
 +
 +
      text-indent: -999em;
 +
 +
      border: 2px solid #fff;
 +
      border-radius: 6px;
 +
 +
      cursor: pointer;
 +
      opacity: .4;
 +
 +
      -webkit-transition: background .5s, opacity .5s;
 +
      -moz-transition: background .5s, opacity .5s;
 +
      transition: background .5s, opacity .5s;
 +
    }
 +
      .banner .dots li.active {
 +
        background: #fff;
 +
        opacity: 1;
 +
      }
 +
 +
  .banner .arrows {
 +
    position: absolute;
 +
    bottom: 20px;
 +
    right: 20px;
 +
    color: #fff;
 +
  }
 +
    .banner .arrow {
 +
      display: inline;
 +
      padding-left: 10px;
 +
      cursor: pointer;
 +
    }
</style>
</style>
-
 
-
<meta name="viewport" content="width=device-width, initial-scale=1.0">
 
-
<meta name="description" content="">
 
-
<meta name="author" content="">
 
-
<title>index</title>
 
</head>
</head>
<body >
<body >
Line 349: Line 302:
</ul>
</ul>
</div>
</div>
-
</div>
+
</div>     
-
<div id="myCarousel" class="carousel slide">
+
<!--the slidershow begin-->
-
<ol class="carousel-indicators">
+
<div class="banner">
-
     <li data-target="#myCarousel" data-slide-to="0"  class="active"></li>
+
       <ul>
-
    <li data-target="#myCarousel" data-slide-to="1"></li>
+
         <li style="background-image: url('http://www.bootcss.com/p/unslider/img/sunset.jpg');">
-
    <li data-target="#myCarousel" data-slide-to="2"></li>
+
           <div class="inner">
-
    <li data-target="#myCarousel" data-slide-to="3"></li>
+
             <h1>Come on! OUC IGEM 2013</h1>
-
  </ol>
+
            <p>不要着急！我们可以弄完的啦～</p>
-
       <div class="carousel-inner">
+
 
-
         <div class="item active">
+
            <a class="btn" href="http://jing.fm/tracks/225c3f.html">来，听首歌轻松下！</a>
-
          <img src="https://static.igem.org/mediawiki/2013/d/df/Ouc-slide-01.jpg" alt="">
+
-
           <div class="container">
+
-
             <div class="carousel-caption">
+
-
              <p class="lead">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p>
+
-
              <a class="btn btn-large btn-primary" href="#">Sign up today</a>
+
-
            </div>
+
           </div>
           </div>
-
         </div>
+
         </li>
-
         <div class="item">
+
 
-
          <img src="https://static.igem.org/mediawiki/2013/2/21/Ouc-slide-02.jpg" alt="">
+
         <li style="background-image: url('http://www.bootcss.com/p/unslider/img/wood.jpg');">
-
           <div class="container">
+
           <div class="inner">
-
             <div class="carousel-caption">
+
             <h1>Come on! OUC IGEM 2013</h1>
-
           
+
            <p>不要着急！我们可以弄完的啦～</p>
-
              <p class="lead">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p>
+
 
-
              <a class="btn btn-large btn-primary" href="#">Learn more</a>
+
            <a class="btn" href="http://jing.fm/tracks/225c3f.html">来，听首歌轻松下！</a>
           </div>
           </div>
-
         </div>
+
         </li>
-
      </div>
+
 
-
         <div class="item">
+
         <li style="background-image: url('img/subway.jpg');">
-
          <img src="https://static.igem.org/mediawiki/2013/f/f2/Ouc-slide-03.jpg" alt="">
+
           <div class="inner">
-
           <div class="container">
+
             <h1>Come on! OUC IGEM 2013</h1>
-
             <div class="carousel-caption">
+
            <p>不要着急！我们可以弄完的啦～</p>
-
             
+
 
-
              <p class="lead">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p>
+
            <a class="btn" href="http://jing.fm/tracks/225c3f.html">来，听首歌轻松下！</a>
-
              <a class="btn btn-large btn-primary" href="#">Browse gallery</a>
+
-
            </div>
+
           </div>
           </div>
-
         </div>
+
         </li>
-
        <div class="item">
+
 
-
          <img src="https://static.igem.org/mediawiki/2013/f/f2/Ouc-slide-02.jpg" alt="">
+
        <li style="background-image: url('img/shop.jpg');">
-
           <div class="container">
+
           <div class="inner">
-
             <div class="carousel-caption">
+
             <h1>Come on! OUC IGEM 2013</h1>
-
           
+
            <p>不要着急！我们可以弄完的啦～</p>
-
              <p class="lead">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p>
+
 
-
              <a class="btn btn-large btn-primary" href="#">Learn more</a>
+
            <a class="btn" href="http://jing.fm/tracks/225c3f.html">来，听首歌轻松下！</a>
           </div>
           </div>
-
         </div>
+
         </li>
-
       </div>
+
       </ul>
-
      </div>
+
  </div>
-
      <a class="left carousel-control" href="#myCarousel" data-slide="prev" data-interval="1000" data-pause="hover">&lsaquo;</a>
+
  <!--the slidershow end-->
-
      <a class="right carousel-control" href="#myCarousel" data-slide="next">&rsaquo;</a>
+
-
    </div>
+
-
   
+
-
   
+
<div class="jumbotron">
<div class="jumbotron">
         <h1>Abstract</h1>
         <h1>Abstract</h1>
Line 429: Line 370:
         <a class="btn btn-large btn-success" href="#">Learn more</a>
         <a class="btn btn-large btn-success" href="#">Learn more</a>
       </div>
       </div>
-
 
       <hr>
       <hr>
-
<script src="http://code.jquery.com/jquery.js"></script>
+
<script src="http://netdna.bootstrapcdn.com/bootstrap/3.0.0/js/bootstrap.min.js"></script>
-
<script src="http://oucast.com/igem/js/bootstrap.min.js"></script>
+
<script src="http://unslider.com/unslider.js"></script>
-
<script>
+
<script>
-
      !function ($) {
+
$(function() {
-
        $(function(){
+
    $('.banner').unslider();
-
          // carousel demo
+
});
-
          $('#myCarousel').carousel()
+
</script>
-
        })
+
<script>
-
       }(window.jQuery)
+
      if(window.chrome) {
 +
        $('.banner li').css('background-size', '100% 100%');
 +
      }
 +
 
 +
       $('.banner').unslider({
 +
        arrows: true,
 +
        fluid: true,
 +
        dots: true
 +
      });     
     </script>
     </script>
-
    <script src="http://oucast.com/igem/js/bootstrap.holder.js"></script>
 
-
 
</body>
</body>
</html>
</html>

Revision as of 11:35, 27 September 2013

Abstract

Putting biological resources into production has now become a hot topic since the development of technology and the draining of natural resources. For example, research about biofuel and biochemistry is now flourishing. But biological products have drawbacks of being inefficient and not broad-spectrum. Inspired by eukaryotic membranous organelles, we aim to construct a prokaryotic membranous organelle to realize division of work inside the cell and improve the efficiency of production. How could a membrane be constructed in a Prokaryote? The answer may lie in this species: Magnetosprillum Magneticum, which can form a natural intracellular membrane. But this bacteria is slow-growing and requires demanding culture conditions, so the purpose of our project is to reconstruct the magnetosome membrane in E.coli, creating better conditions for efficient biological production.

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Specialist of our project

1.We designed an artificial prokaryotic membranous organelle which is capable of anchoring proteins, opening up new possibilities for intracellular biochemistry reactions.
2.We took advantage of the 3D structure of RNA, using ribosomes as a barrier to stabilize RNA.
3.We used Microfluidic Technology to detect the magnetism of our magnetic bacteria, Magnetospirillum Magneticum.
4.We preserved Magnetospirillum Magneticum AMB-1 mamAB genes in E.coli, prevented the genes lose when AMB-1 strain was cultured in High oxygen partial pressure environment.

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Reconstructing the Magnetosome Membrane in E. coli

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