Team:OUC-China

From 2013.igem.org

(Difference between revisions)
 
(111 intermediate revisions not shown)
Line 1: Line 1:
<html lang="en">
<html lang="en">
<head>
<head>
-
<meta http-equiv="Content-Type" content="text/html; charset=gb2312" />
+
<meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">
-
<link href="http://oucast.com\igem\css\bootstrap.css" rel="stylesheet" media="screen">
+
<meta name="viewport" content="width=device-width, initial-scale=1.0">
-
<link href="http://oucast.com\igem\css\bootstrap-responsive.css" rel="stylesheet" media="screen">
+
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
-
 
+
<link href="http://netdna.bootstrapcdn.com/bootstrap/3.0.0/css/bootstrap.min.css" rel="stylesheet" media="screen">
-
 
+
<script src="http://code.jquery.com/jquery.min.js"></script>
<style>
<style>
-
body {
+
/*the igem Global setting*/
-
font: x-small/normal sans-serif; margin: 0px; padding: 0px; color: black; font-size-adjust: none; font-stretch: normal; background-color: rgb(252, 241, 206);}
+
* {
-
.visualClear {
+
  margin: 0;
-
clear: both;
+
  padding: 0;
 +
  -webkit-font-smoothing: antialiased;
 +
  -webkit-box-sizing: border-box;
 +
  -moz-box-sizing: border-box;
 +
  box-sizing: border-box;
 +
}
 +
body{
 +
  color: #34495e;
 +
}
 +
#bodyContent h1, #bodyContent h2{
 +
  margin-bottom:0;
 +
}
 +
h1, h2, h3, h4, h5, h6{
 +
  padding-top: 0;
 +
  padding-bottom: 0;
 +
  color: #34495e;
}
}
#globalWrapper {
#globalWrapper {
-
margin: 0px; padding: 0px 0px 0px; width: auto; font-size: 127%; position: relative;
+
  font-size: 100%;
-
#top-section {
+
-
margin-top:0;
+
-
        margin-left:0;
+
-
        width: 0px;
+
-
        height: 0px;
+
-
        height: 0px !important;
+
-
        position: relative;
+
-
        z-index: 100;
+
-
        border: 0px;
+
-
        border: 0px !important;
+
}
}
-
#p-logo {
+
#top-section{
-
        visibility:hidden;
+
  height:0px;
-
width: 975px; height: 0; position: absolute; z-index: -1;
+
    border:none;
 +
    width:980px;
 +
    margin:0 auto;
 +
    padding:0 0 0 0;
 +
    background-color:transparent;
 +
    overflow:hide;
}
}
-
#p-logo h5 {
+
#p-logo{display:none;}
-
display: none;
+
#search-controls{display:none;}
 +
#top{display:none;}
 +
.firstHeading{display:none;}
 +
#footer-box{display:none;}
 +
#catlinks{display:none;}
 +
div#contentSub{
 +
    display: none;
 +
    position: absolute;
}
}
-
#p-logo a {
+
#content h1.firstHeading {display: none;}
-
text-decoration: none;
+
#content
 +
{
 +
  border:none;
 +
  height: 100%;
 +
  margin:0;
 +
  padding:0;
 +
  width:100%;
 +
  position: absolute;
 +
  background-color: #FFFFFF;
 +
  color: #34495e;
}
}
-
#p-logo a:hover {
+
a:visited{
-
text-decoration: none;
+
  color: #fff;
 +
}
 +
/*the nav settings
 +
----------------------------------------------- */
 +
.navbar-inverse .navbar-nav>.open>a, .navbar-inverse .navbar-nav>.open>a:hover, .navbar-inverse .navbar-nav>.open>a:focus{
 +
  color: #1abc9c;
 +
  background-color: transparent;
 +
}
 +
.navbar-inverse .navbar-nav>li>a{
 +
  color: #fff;
 +
}
 +
.navbar-inverse .navbar-nav>li>a:hover{
 +
  color: #1abc9c;
 +
  background-color:transparent;
 +
}
 +
.navbar-inverse .navbar-brand{
 +
          color: #fff;
 +
          padding: 0;
 +
}
 +
.navbar-inverse .navbar-brand img{
 +
  height: 50px;
 +
  padding-left: 90px;
 +
}
 +
.navbar-inverse .navbar-brand p{
 +
  padding:12px 30px;
 +
  margin:0;
 +
}
 +
.navbar-inverse .navbar-brand:hover{
 +
  color: #1abc9c;
 +
}
 +
.navbar-inverse{
 +
  border-color: transparent;
 +
  background: #34495e;
 +
  font-weight: bold;
 +
  filter: progid:DXImageTransform.Microsoft.gradient(enabled = false);
 +
}
 +
.dropdown-menu{
 +
  background-color:#34495e;
 +
  border:none;
 +
  -webkit-box-shadow:none;
 +
  box-shadow: none;
 +
}
 +
.dropdown-menu>li>a{
 +
  color: #fff;
 +
  font-weight: bold;
 +
}
 +
.dropdown-menu>li>a:hover, .dropdown-menu>li>a:focus{
 +
  color:#1abc9c;
 +
  background-color: transparent;
}
}
-
/* GLOBAL STYLES
+
/*add dropdown-submenu to btV3*/
-
    -------------------------------------------------- */
+
.dropdown-submenu{position:relative;}
-
    /* Padding below the footer and lighter body text */
+
.dropdown-submenu>.dropdown-menu{top:0;left:100%;margin-top:-6px;margin-left:-1px;-webkit-border-radius:0 6px 6px 6px;-moz-border-radius:0 6px 6px 6px;border-radius:0 6px 6px 6px;}
 +
.dropdown-submenu:hover>.dropdown-menu{display:block;}
 +
.dropdown-submenu>a:after{display:block;content:" ";float:right;width:0;height:0;border-color:transparent;border-style:solid;border-width:5px 0 5px 5px;border-left-color:#cccccc;margin-top:5px;margin-right:-10px;}
 +
.dropdown-submenu:hover>a:after{border-left-color:#ffffff;}
 +
.dropdown-submenu.pull-left{float:none;}.dropdown-submenu.pull-left>.dropdown-menu{left:-100%;margin-left:10px;-webkit-border-radius:6px 0 6px 6px;-moz-border-radius:6px 0 6px 6px;border-radius:6px 0 6px 6px;}
 +
/*the unslider css*/
 +
.banner {
 +
  position: relative;
 +
  width: 100%;
 +
  overflow: auto;
 +
  font-size: 18px;
 +
  line-height: 24px;
 +
  text-align: center;
 +
  color: #fff;
 +
  background: ;
 +
  box-shadow: 0 1px 2px rgba(0,0,0,.25);
 +
}
 +
  .banner ul {
 +
    list-style: none;
 +
    width: 300%;
 +
    margin: 0;
 +
  }
 +
  .banner ul li {
 +
    display: block;
 +
    float: left;
 +
    width: 33%;
 +
    min-height: 350px;
-
     h1 {
+
     -o-background-size: 100% 100%;
-
color:#0099FF;
+
     -ms-background-size: 100% 100%;
-
}
+
    -moz-background-size: 100% 100%;
-
     body {
+
    -webkit-background-size: 100% 100%;
-
      padding-bottom: 40px;
+
     background-size: 100% 100%;
-
      color: #5a5a5a;
+
-
  background: #e5e5e5 url(image/moon.png);
+
-
  overflow-x: hidden;
+
-
  -webkit-user-select: none;
+
-
  -webkit-tap-highlight-color: rgba(0,0,0,0);
+
-
     }
+
-
     .jumbotron {
+
     box-shadow: inset 0 -3px 6px rgba(0,0,0,.1);
-
        margin: 60px 0;
+
  }
-
        text-align: center;
+
-
      }
+
-
      .jumbotron h1 {
+
-
        font-size: 72px;
+
-
        line-height: 1;
+
-
      }
+
-
      .jumbotron .btn {
+
-
        font-size: 21px;
+
-
        padding: 14px 24px;
+
-
      }
+
-
      /* Supporting marketing content */
+
  .banner .inner {
-
      .marketing {
+
-
        margin: 60px 0;
+
-
      }
+
-
      .marketing p + h4 {
+
-
        margin-top: 28px;
+
-
      }
+
-
    /* CUSTOMIZE THE NAVBAR
 
-
    -------------------------------------------------- */
 
-
     /* Special class on .container surrounding .navbar, used for positioning it into place. */
+
     padding: 160px 0 110px;
-
    .navbar-wrapper {
+
  }
-
      position: absolute;
+
-
      top: 0;
+
-
      left: 0;
+
-
      right: 0;
+
-
      z-index: 10;
+
-
      margin-top: 20px;
+
-
      margin-bottom: -90px; /* Negative margin to pull up carousel. 90px is roughly margins and height of navbar. */
+
-
    }
+
-
    .navbar-wrapper .navbar {
+
-
     }
+
  .banner h1, .banner h2 {
 +
     font-size: 40px;
 +
    line-height: 52px;
 +
    border-bottom:#fff;
 +
    color: #fff;
 +
  }
-
    /* Remove border and change up box shadow for more contrast */
+
  .banner .btn {
-
    .navbar .navbar-inner {
+
    display: inline-block;
-
      border: 0;
+
    margin: 25px 0 0;
-
      -webkit-box-shadow: 0 2px 20px rgba(10,0,0,.25);
+
    padding: 9px 22px 7px;
-
        -moz-box-shadow: 0 2px 20px rgba(10,0,0,.25);
+
    clear: both;
-
              box-shadow: 0 2px 20px rgba(10,0,0,.25);
+
-
    }
+
-
     /* Downsize the brand/project name a bit */
+
     color: #fff;
-
    .navbar .brand {
+
    font-size: 12px;
-
      padding: 14px 20px 16px; /* Increase vertical padding to match navbar links */
+
    font-weight: bold;
-
      font-size: 16px;
+
    text-transform: uppercase;
-
      font-weight: bold;
+
     text-decoration: none;
-
      text-shadow: 0 -1px 0 rgba(0,0,0,.5);
+
-
     }
+
-
     /* Navbar links: increase padding for taller navbar */
+
     border: 2px solid rgba(255,255,255,.4);
-
     .navbar .nav > li > a {
+
    border-radius: 5px;
-
       padding: 15px 20px;
+
  }
 +
     .banner .btn:hover {
 +
       background: rgba(255,255,255,.05);
     }
     }
-
 
+
     .banner .btn:active {
-
    /* Offset the responsive button for proper vertical alignment */
+
       -webkit-filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
-
     .navbar .btn-navbar {
+
      -moz-filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
-
       margin-top: 10px;
+
      -ms-filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
 +
      -o-filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
 +
      filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
     }
     }
 +
  .banner .btn, .banner .dot {
 +
    -webkit-filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
 +
    -moz-filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
 +
    -ms-filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
 +
    -o-filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
 +
    filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
 +
  }
 +
  .banner .dots {
 +
    position: absolute;
 +
    left: 0;
 +
    right: 0;
 +
    bottom: 20px;
 +
    margin: 0;
 +
  }
 +
    .banner .dots li {
 +
      display: inline-block;
 +
      width: 10px;
 +
      height: 10px;
 +
      margin: 0 4px;
-
    /* CUSTOMIZE THE CAROUSEL
+
      text-indent: -999em;
-
    -------------------------------------------------- */
+
-
    /* Carousel base class */
+
      border: 2px solid #fff;
-
    .carousel {
+
       border-radius: 6px;
-
       margin-bottom: 60px;
+
-
    }
+
-
    .carousel .container {
+
       cursor: pointer;
-
       position: relative;
+
       opacity: .4;
-
       z-index: 9;
+
-
    }
+
-
    .carousel-control {
+
       -webkit-transition: background .5s, opacity .5s;
-
       height: 80px;
+
       -moz-transition: background .5s, opacity .5s;
-
      margin-top: 0;
+
       transition: background .5s, opacity .5s;
-
      font-size: 120px;
+
-
       text-shadow: 0 1px 1px rgba(0,0,0,.4);
+
-
       background-color: transparent;
+
-
      border: 0;
+
-
      z-index: 10;
+
     }
     }
-
 
+
      .banner .dots li.active {
-
    .carousel .item {
+
        background: #fff;
-
      height: 400px;
+
        opacity: 1;
-
    }
+
-
    .carousel img {
+
-
      position: absolute;
+
-
      top: 0;
+
-
      left: 0;
+
-
      min-width: 100%;
+
-
      height: 400px;
+
-
    }
+
-
 
+
-
    .carousel-caption {
+
-
      background-color: transparent;
+
-
      position: static;
+
-
      max-width: 550px;
+
-
      padding: 0 20px;
+
-
      margin-top: 200px;
+
-
    }
+
-
    .carousel-caption h1,
+
-
    .carousel-caption .lead {
+
-
      margin: 0;
+
-
      line-height: 1.25;
+
-
      color: #fff;
+
-
      text-shadow: 0 1px 1px rgba(0,0,0,.4);
+
-
    }
+
-
    .carousel-caption .btn {
+
-
      margin-top: 10px;
+
-
    }
+
-
 
+
-
 
+
-
 
+
-
    /* MARKETING CONTENT
+
-
    -------------------------------------------------- */
+
-
 
+
-
    /* Center align the text within the three columns below the carousel */
+
-
    .marketing .span6 {
+
-
      text-align: center;
+
-
    }
+
-
    .marketing h2 {
+
-
      font-weight: normal;
+
-
      margin-left: 200px;
+
-
      margin-right: 60px;
+
-
    }
+
-
    .marketing .span6 p {
+
-
      margin-left: 200px;
+
-
      margin-right: 60px;
+
-
    }
+
-
 
+
-
 
+
-
    /* Featurettes
+
-
    ------------------------- */
+
-
 
+
-
    .featurette-divider {
+
-
      margin: 80px 0; /* Space out the Bootstrap <hr> more */
+
-
    }
+
-
    .featurette {
+
-
      padding-top: 120px; /* Vertically center images part 1: add padding above and below text. */
+
-
      overflow: hidden; /* Vertically center images part 2: clear their floats. */
+
-
    }
+
-
    .featurette-image {
+
-
      margin-top: -120px; /* Vertically center images part 3: negative margin up the image the same amount of the padding to center it. */
+
-
    }
+
-
 
+
-
    /* Give some space on the sides of the floated elements so text doesn't run right into it. */
+
-
    .featurette-image.pull-left {
+
-
      margin-right: 40px;
+
-
    }
+
-
    .featurette-image.pull-right {
+
-
      margin-left: 40px;
+
-
    }
+
-
 
+
-
    /* Thin out the marketing headings */
+
-
    .featurette-heading {
+
-
      font-size: 50px;
+
-
      font-weight: 300;
+
-
      line-height: 1;
+
-
      letter-spacing: -1px;
+
-
    }
+
-
 
+
-
 
+
-
 
+
-
    /* RESPONSIVE CSS
+
-
    -------------------------------------------------- */
+
-
 
+
-
    @media (max-width: 979px) {
+
-
 
+
-
      .container.navbar-wrapper {
+
-
        margin-bottom: 0;
+
-
        width: auto;
+
-
      }
+
-
      .navbar-inner {
+
-
        border-radius: 0;
+
-
        margin: -20px 0;
+
       }
       }
-
      .carousel .item {
+
  .banner .arrows {
-
        height: 400px;
+
    position: absolute;
-
      }
+
    bottom: 20px;
-
      .carousel img {
+
    right: 20px;
-
        width: auto;
+
    color: #fff;
-
        height: 400px;
+
  }
-
      }
+
    .banner .arrow {
-
 
+
       display: inline;
-
      .featurette {
+
      padding-left: 10px;
-
        height: auto;
+
       cursor: pointer;
-
      }
+
-
      .featurette-image.pull-left,
+
-
       .featurette-image.pull-right {
+
-
        display: block;
+
-
        float: none;
+
-
        max-width: 40%;
+
-
        margin: 0 auto 20px;
+
-
       }
+
-
        padding: 0;
+
     }
     }
 +
/*features*/
 +
.features {
 +
  overflow: hidden;
 +
  padding: 50px 0;
 +
}
 +
  .features li {
 +
    position: relative;
 +
    padding: 0 25px 0 40px;
 +
    list-style: none;
-
     @media (max-width: 767px) {
+
     width: 25%;
 +
    float: left;
 +
  }
 +
    .features li:before {
 +
      content: '';
 +
      position: absolute;
 +
      left: 0;
 +
      top: 0;
-
       .navbar-inner {
+
       display: block;
-
        margin: -20px;
+
       width: 24px;
-
       }
+
       height: 22px;
-
 
+
-
      .carousel {
+
-
        margin-left: -20px;
+
-
        margin-right: -20px;
+
-
      }
+
-
      .carousel .container {
+
-
 
+
-
      }
+
-
      .carousel .item {
+
-
        height: 400px;
+
-
      }
+
-
      .carousel img {
+
-
        height: 400px;
+
-
      }
+
-
      .carousel-caption {
+
-
        width: 65%;
+
-
        padding: 0 70px;
+
-
        margin-top: 100px;
+
-
       }
+
-
      .carousel-caption h1 {
+
-
        font-size: 30px;
+
-
      }
+
-
      .carousel-caption .lead,
+
-
      .carousel-caption .btn {
+
-
        font-size: 18px;
+
-
      }
+
-
 
+
-
      .marketing .span4 + .span4 {
+
-
        margin-top: 40px;
+
-
      }
+
-
 
+
-
      .featurette-heading {
+
-
        font-size: 30px;
+
-
      }
+
-
      .featurette .lead {
+
-
        font-size: 18px;
+
-
        line-height: 1.5;
+
-
      }
+
 +
      background: url('img/icons.png');
     }
     }
-
+
      .features li.browser:before { background-position: 0 -44px; }
-
/* = 通用 Global
+
      .features li.height:before { background-position: 0 -22px; }
-
----------------------------------------------- */
+
      .features li.responsive:before { background-position: 0 -66px; }
-
a:not(.btn) {
+
  .features b {
-
color: #00699E;
+
    display: block;
-
-webkit-transition: 300ms;
+
    padding-bottom: 6px;
-
-moz-transition: 300ms;
+
  }
-
transition: 300ms;
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            <h1>WE DID IT !</h1>
 +
            <p>Constructing an Artificial Organelle（Intracellular Compartment） in <i>E.coli</i></p>
 +
            <a class="btn" href="https://2013.igem.org/Team:OUC-China/Results">learn the story behind this picture</a>
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            <h1>Compression of Metabolic Pathways</h1>
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            <p>Constructing an artificial cluster to build the compartment by operating fewer genes. </p>
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            <a class="btn" href="https://2013.igem.org/Team:OUC-China/Design">learn more</a>
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            <h1>Microfluidic Chip Magnetism Detection</h1>
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            <h1>Factors Considered in RNA guardian</h1>
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            <p><br /></p>
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            <a class="btn" href="https://2013.igem.org/Team:OUC-China/RNA_guardian/Design">Learn more</a>
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      <h3><b>Features Of Our Project</b></h3><hr>
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        <li>
 +
          <b><span class="glyphicon glyphicon-ok-sign"></span> Artificial Organelle(Intracellular Compartment)</b>
 +
          <p>We designed an artificial prokaryotic membranous organelle which is capable of anchoring proteins, opening up new possibilities for intracellular biochemistry reactions.</p>
 +
          <a class="btn btn-info" href="https://2013.igem.org/Team:OUC-China/Results">Learn more</a>
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          <b><span class="glyphicon glyphicon-ok-sign"></span> RNA Guardian</b>
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          <p>We took advantage of the 3D structure of RNA, using ribosomes as a barrier to stabilize RNA.</p>
 +
          <a class="btn btn-info" href="https://2013.igem.org/Team:OUC-China/Instruction">Learn more</a>
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        <li>
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          <b><span class="glyphicon glyphicon-ok-sign"></span> Microfluidic</b>
 +
          <p>We used Microfluidic Technology to detect the magnetism of our magnetic bacteria, <i>Magnetospirillum Magneticum</i>.</p>
 +
          <a class="btn btn-info" href="https://2013.igem.org/Team:OUC-China/Microfluidics">Learn more</a>
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        </li>
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        <li>
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          <b><span class="glyphicon glyphicon-ok-sign"></span> Preserving <i>mam</i>AB genes</b>
 +
          <p>We preserved <i>Magnetospirillum Magneticum</i> AMB-1 <i>mam</i>AB genes in <i>E.coli</i>, prevented the genes lose when AMB-1 strain was cultured in high oxygen partial pressure environment.</p>
 +
          <a class="btn btn-info" href="https://2013.igem.org/Team:OUC-China/Design">Learn more</a>
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        <h3><b>Introduction</b></h3><hr>
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        <p>Putting biological resources into production has now become a hot topic since the development of technology and the draining of natural resources. For example, research about biofuel and biochemistry is now flourishing. But biological products have drawbacks of being inefficient and not broad-spectrum. Inspired by eukaryotic membranous organelles, we aim to construct a prokaryotic membranous organelle to realize division of work inside the cell and improve the efficiency of production.
 +
How could a membrane be constructed in a Prokaryote? The answer may lie in this species: <i>Magnetosprillum Magneticum</i>, which can form a natural intracellular membrane. But this bacteria is slow-growing and requires demanding culture conditions, so the purpose of our project is to reconstruct the magnetosome membrane in <i><i>E.coli</i></i>, creating better conditions for efficient biological production.</p>
 +
        <p style="text-align:center"><a class="btn btn-large btn-info" href="https://2013.igem.org/Team:OUC-China/Overview">Learn more</a></p>
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Latest revision as of 03:38, 28 September 2013

    Features Of Our Project

  • Artificial Organelle(Intracellular Compartment)

    We designed an artificial prokaryotic membranous organelle which is capable of anchoring proteins, opening up new possibilities for intracellular biochemistry reactions.

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  • RNA Guardian

    We took advantage of the 3D structure of RNA, using ribosomes as a barrier to stabilize RNA.

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  • Microfluidic

    We used Microfluidic Technology to detect the magnetism of our magnetic bacteria, Magnetospirillum Magneticum.

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  • Preserving mamAB genes

    We preserved Magnetospirillum Magneticum AMB-1 mamAB genes in E.coli, prevented the genes lose when AMB-1 strain was cultured in high oxygen partial pressure environment.

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Introduction

Putting biological resources into production has now become a hot topic since the development of technology and the draining of natural resources. For example, research about biofuel and biochemistry is now flourishing. But biological products have drawbacks of being inefficient and not broad-spectrum. Inspired by eukaryotic membranous organelles, we aim to construct a prokaryotic membranous organelle to realize division of work inside the cell and improve the efficiency of production. How could a membrane be constructed in a Prokaryote? The answer may lie in this species: Magnetosprillum Magneticum, which can form a natural intracellular membrane. But this bacteria is slow-growing and requires demanding culture conditions, so the purpose of our project is to reconstruct the magnetosome membrane in E.coli, creating better conditions for efficient biological production.

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