Team:OUC-China/Introduction

From 2013.igem.org

(Difference between revisions)
Line 3: Line 3:
<meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">
<meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
-
<meta http-equiv="Content-Type" content="text/html; charset=gb2312" />
+
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
-
<link href="http://oucast.com/igem/css/bootstrap.css" rel="stylesheet" media="screen">
+
<link href="http://netdna.bootstrapcdn.com/bootstrap/3.0.0/css/bootstrap.min.css" rel="stylesheet" media="screen">
-
<link href="http://oucast.com/igem/css/bootstrap-responsive.css" rel="stylesheet" media="screen">
+
-
<link href="http://oucast.com/igem/css/docs.css" rel="stylesheet">
+
-
 
+
<style type="text/css">
<style type="text/css">
-
 
+
/*the igem Global setting*/
-
 
+
* {
 +
  margin: 0;
 +
  padding: 0;
 +
  -webkit-font-smoothing: antialiased;
 +
  -webkit-box-sizing: border-box;
 +
  -moz-box-sizing: border-box;
 +
  box-sizing: border-box;
 +
}
 +
#menubar{
 +
  display: none;
 +
}
 +
body{
 +
  color: #34495e;
 +
  padding-top: 80px;
 +
}
 +
#bodyContent h1, #bodyContent h2{
 +
  margin-bottom:0;
 +
}
 +
h1, h2, h3, h4, h5, h6{
 +
  padding-top: 0;
 +
  padding-bottom: 0;
 +
  color: #34495e;
 +
}
 +
#globalWrapper {
 +
  font-size: 100%;
 +
}
#top-section{
#top-section{
   height:0px;
   height:0px;
Line 17: Line 39:
     margin:0 auto;
     margin:0 auto;
     padding:0 0 0 0;
     padding:0 0 0 0;
-
     background-color:#e5e5e5;
+
     background-color:transparent;
     overflow:hide;
     overflow:hide;
-
 
}
}
#p-logo{display:none;}
#p-logo{display:none;}
Line 34: Line 55:
#content
#content
{
{
-
border:none;
+
  border:none;
-
height: 100%;
+
  height: 100%;
-
margin:0;
+
  margin:0;
-
padding:0;
+
  padding:0;
-
width:100%;
+
  width:100%;
-
position: absolute;
+
  position: absolute;
-
background-color: #e5e5e5;
+
  background-color: #FFFFFF;
 +
  color: #34495e;
}
}
-
 
+
a:visited{
-
{
+
  color: #fff;
-
margin:0;
+
-
padding:0;
+
}
}
-
 
+
/*the nav settings
-
 
+
----------------------------------------------- */
-
#menubar {
+
.navbar-inverse .navbar-nav>.open>a, .navbar-inverse .navbar-nav>.open>a:hover, .navbar-inverse .navbar-nav>.open>a:focus{
-
    background-color:transparent;
+
  color: #1abc9c;
-
    position: relative;
+
  background-color: transparent;
-
    float:left;
+
-
    white-space: nowrap;
+
-
    top:-6px;
+
-
    width: 490px;
+
-
    z-index: 5000;
+
-
    font-family: sans-serif;
+
-
    font-size: 50%;
+
-
    line-height: 1em;
+
-
    z-index:99;
+
}
}
-
li {font-weight:normal;
+
.navbar-inverse .navbar-nav>li>a{
-
    font-size:12px;
+
  color: #fff;
 +
}
 +
.navbar-inverse .navbar-nav>li>a:hover{
 +
  color: #1abc9c;
 +
  background-color:transparent;
 +
}
 +
.navbar-inverse .navbar-brand{
 +
          color: #fff;
 +
          padding: 0;
 +
}
 +
.navbar-inverse .navbar-brand img{
 +
  height: 50px;
 +
  padding-left: 90px;
 +
}
 +
.navbar-inverse .navbar-brand p{
 +
  padding:12px 30px;
 +
  margin:0;
 +
}
 +
.navbar-inverse .navbar-brand:hover{
 +
  color: #1abc9c;
 +
}
 +
.navbar-inverse{
 +
  border-color: transparent;
 +
  background: #34495e;
 +
  font-weight: bold;
 +
  filter: progid:DXImageTransform.Microsoft.gradient(enabled = false);
 +
}
 +
.dropdown-menu{
 +
  background-color:#34495e;
 +
  border:none;
 +
  -webkit-box-shadow:none;
 +
  box-shadow: none;
 +
}
 +
.dropdown-menu>li>a{
 +
  color: #fff;
 +
  font-weight: bold;
 +
}
 +
.dropdown-menu>li>a:hover, .dropdown-menu>li>a:focus{
 +
  color:#1abc9c;
 +
  background-color: transparent;
}
}
-
body {
+
/*add dropdown-submenu to btV3*/
-
      padding-bottom: 40px;
+
.dropdown-submenu{position:relative;}
-
      color: #5a5a5a;
+
.dropdown-submenu>.dropdown-menu{top:0;left:100%;margin-top:-6px;margin-left:-1px;-webkit-border-radius:0 6px 6px 6px;-moz-border-radius:0 6px 6px 6px;border-radius:0 6px 6px 6px;}
-
  background: #e5e5e5 url(bootstrap/img/moon.png);
+
.dropdown-submenu:hover>.dropdown-menu{display:block;}
-
  overflow-x: hidden;
+
.dropdown-submenu>a:after{display:block;content:" ";float:right;width:0;height:0;border-color:transparent;border-style:solid;border-width:5px 0 5px 5px;border-left-color:#cccccc;margin-top:5px;margin-right:-10px;}
-
  -webkit-user-select: none;
+
.dropdown-submenu:hover>a:after{border-left-color:#ffffff;}
-
  -webkit-tap-highlight-color: rgba(0,0,0,0);
+
.dropdown-submenu.pull-left{float:none;}.dropdown-submenu.pull-left>.dropdown-menu{left:-100%;margin-left:10px;-webkit-border-radius:6px 0 6px 6px;-moz-border-radius:6px 0 6px 6px;border-radius:6px 0 6px 6px;}
-
    }
+
-
h1 {color:#0099FF}
+
 +
/*footer*/
 +
footer{
 +
  margin-top: 100px;
 +
  background-color: #eceff1;
 +
  color: #bdc1c5;
 +
  font-size: 17px;
 +
  padding: 0;
}
}
-
 
+
.footer-banner{
-
a:not(.btn) {
+
  background-color: #34495e;
-
color: #00699E;
+
  color: #58F8D9;
-
-webkit-transition: 300ms;
+
  margin-left: 42px;
-
-moz-transition: 300ms;
+
  min-height: 286px;
-
transition: 300ms;
+
  padding: 0 30px 30px;
}
}
-
a:hover:not(.btn),
+
.footer-banner ul{
-
a:active:not(.btn) {
+
  list-style-type: none;
-
color: #9E0000;
+
-
text-decoration: none;
+
-
-webkit-transform: translateY(-8px);
+
-
-moz-transform: translateY(-8px);
+
-
transform: translateY(-8px);
+
}
}
-
 
+
.footer-banner ul li{
-
hr.soften {
+
  border-bottom:1px solid rgba(57, 107, 97, 0.53);
-
height: 1px;
+
  line-height: 19px;
-
margin: 30px 0;
+
  padding: 6px 0;
-
background-image: -webkit-linear-gradient(left, rgba(0,0,0,0), rgba(0,0,0,.1), rgba(0,0,0,0));
+
-
background-image:   -moz-linear-gradient(left, rgba(0,0,0,0), rgba(0,0,0,.1), rgba(0,0,0,0));
+
-
background-image:    -ms-linear-gradient(left, rgba(0,0,0,0), rgba(0,0,0,.1), rgba(0,0,0,0));
+
-
background-image:      -o-linear-gradient(left, rgba(0,0,0,0), rgba(0,0,0,.1), rgba(0,0,0,0));
+
-
border: 0;
+
}
}
-
 
+
.footer-banner a{
-
.nav a {
+
  color: #58F8D9;
-
-webkit-transition: 500ms;
+
}
-
-moz-transition: 500ms;
+
.footer-banner a:hover{
-
transition: 500ms;
+
  text-decoration: none;
 +
}
 +
.footer-title{
 +
  margin: 0 0 22px;
 +
  padding-top: 21px;
 +
  color: #1abc9c;
 +
  font-weight: bold;
 +
}
 +
.footerleft a{
 +
  color: #bdc1c5;
 +
}
 +
.footerleft a:visited {
 +
  color: #bdc1c5;
}
}
 +
/*slide nav*/
 +
.bs-docs-sidebar{
 +
  margin-bottom: 30px;
 +
  padding-top: 10px;
 +
  padding-bottom: 10px;
 +
}
 +
.bs-docs-sidebar a{
 +
  color: #34495e;
 +
}
 +
.bs-docs-sidebar a:hover{
 +
  color: #16A085;
 +
}
</style>
</style>
<meta name="viewport" content="width=device-width, initial-scale=1.0">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
  <meta name="description" content="">
  <meta name="description" content="">
  <meta name="author" content="">
  <meta name="author" content="">
-
<title>Introduction</title>
+
<title>Overview</title>
</head>
</head>
-
 
<body>
<body>
-
<div class="navbar navbar-inverse navbar-fixed-top">
+
<div class="navbar navbar-inverse navbar-fixed-top" role="navigation">
-
      <div class="navbar-inner">
+
  <div class="navbar-header">
-
        <div class="container">
+
    <button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-ex1-collapse">
-
<div class="row"><div class="span3"><a href="https://2013.igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2013/6/6a/Ouc-igem1.jpg" alt="logo" style="width:70px;height:50px;margin-top:0px;padding:0 0 0 0;" class="brand png"></a></div>
+
      <span class="sr-only">The navigation</span>
-
          <button type="button" class="btn btn-navbar" data-toggle="collapse" data-target=".nav-collapse">
+
          <span class="icon-bar"></span>
-
            <span class="icon-bar"></span>
+
          <span class="icon-bar"></span>
-
            <span class="icon-bar"></span>
+
          <span class="icon-bar"></span>
-
            <span class="icon-bar"></span>
+
        </button>
-
          </button>
+
    <a class="navbar-brand" href="https://igem.org/Team_Wikis?year=2013">
-
            <div class="nav-collapse collapse">
+
      <img src="https://static.igem.org/mediawiki/2013/7/7d/Ouc-igem1.png">
-
                  <ul class="nav pull-right">
+
    </a>
-
  <li class="active"><a href="https://2013.igem.org/Team:OUC-China"><font size="4.5px">Home</font></a></li>
+
    <a class="navbar-brand" href="https://2013.igem.org/Team:OUC-China">
-
              <li class="dropdown">
+
      <p>OUC-China IGEM 2013</p>
-
                <a class="dropdown-toggle" id="drop4" role="button" data-toggle="dropdown" href="#"><font size="4.5px">Project</font><b class="caret"></b>
+
    </a>
-
</a>
+
  </div>
-
                <ul id="menu1" class="dropdown-menu" role="menu" aria-labelledby="drop4">
+
    <div class="collapse navbar-collapse navbar-ex1-collapse">
-
<li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Overview">Overview</a>
+
      <ul class="nav navbar-nav pull-right">
-
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Achievement">Achievement & judge criteria</a>
+
      <li><a href="https://2013.igem.org/Team:OUC-China">Home</a></li>
-
                  <li class="dropdown-submenu">
+
            <li class="dropdown" role="menu" aria-labelledby="dropdownMenu">
-
                  <a tabindex="-1" href="#">Intercellular compartment</a>
+
              <a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<b class="caret"></b></a>
-
  <ul class="dropdown-menu">
+
              <ul class="dropdown-menu">
-
                      <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Review">Review</a></li>
+
          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Overview">Overview</a></li>
-
                      <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Design">Design</a></li>
+
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Achievement">Achievement & judge criteria</a></li>
-
  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Microfluidics">Microfluidics</a></li>
+
                    <li class="dropdown-submenu">
-
  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Results">Results</a></li>
+
                      <a tabindex="-1" href="#">Intracellular compartment</a>
-
 
+
                <ul class="dropdown-menu">
-
  </ul>
+
                        <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Review">Review</a></li>
-
  </li>
+
                        <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Design">Design</a></li>
 +
              <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Microfluidics">Microfluidics</a></li>
 +
              <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Results">Results</a></li>
 +
                </ul>
 +
            </li>
                   <li class="dropdown-submenu">
                   <li class="dropdown-submenu">
                   <a tabindex="-1" href="#">RNA guardian</a>
                   <a tabindex="-1" href="#">RNA guardian</a>
-
  <ul class="dropdown-menu">
+
          <ul class="dropdown-menu">
                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Introduction">Introduction</a></li>
                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Introduction">Introduction</a></li>
                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Design">Design</a></li>
                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Design">Design</a></li>
-
  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Results">Result</a></li>
+
            <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Results">Result</a></li>
-
  <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Reference">Reference</a></li>
+
            <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Reference">Reference</a></li>
-
 
+
            </ul>
-
 
+
          </li>
-
  </ul>
+
          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Part description">Part description</a></li>
-
  </li>
+
          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Future work">Future work</a></li>
-
  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Part description">Part description</a></li>
+
-
                  <li role="presentation" class="divider"></li>
+
-
  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Future work">Future work</a></li>
+
                 </ul>
                 </ul>
-
              </li>
+
            </li>
-
              <li class="dropdown">
+
            <li class="dropdown" role="menu" aria-labelledby="dropdownMenu">
-
                <a class="dropdown-toggle" id="drop5" role="button" data-toggle="dropdown" href="#"><font size="4.5px">Modeling</font><b class="caret"></b></a>
+
              <a class="dropdown-toggle" data-toggle="dropdown" href="#">Modeling<b class="caret"></b></a>
-
                 <ul id="menu2" class="dropdown-menu" role="menu" aria-labelledby="drop5">
+
                 <ul class="dropdown-menu">
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Magnetic Analysis">Magnetic Analysis</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Magnetic Analysis">Magnetic Analysis</a></li>
-
                  <li role="presentation" class="divider"></li>
 
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Model in RNA guardian">Model in RNA guardian</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Model in RNA guardian">Model in RNA guardian</a></li>
                 </ul>
                 </ul>
-
              </li>
+
            </li>
-
              <li class="dropdown">
+
            <li><a  href="https://2013.igem.org/Team:OUC-China/Human Practice">Human Practice</a></li>
-
                <a  href="https://2013.igem.org/Team:OUC-China/Human Practice"><font size="4.5px">Human Practice</font></a>
+
      <li class="dropdown">
-
               
+
              <a class="dropdown-toggle" data-toggle="dropdown" href="#">Team<b class="caret"></b></a>
-
              </li>
+
              <ul class="dropdown-menu" role="menu" aria-labelledby="dropdownMenu">
-
<li class="dropdown">
+
-
                <a class="dropdown-toggle" id="drop5" role="button" data-toggle="dropdown" href="#"><font size="4.5px">Team</font><b class="caret"></b></a>
+
-
              <ul id="menu2" class="dropdown-menu" role="menu" aria-labelledby="drop5">
+
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Members">Members</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Members">Members</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Instructors">Instructors</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Instructors">Instructors</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Advisor">Advisor</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Advisor">Advisor</a></li>
-
  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Lab">Lab</a></li>
+
          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Lab">Lab</a></li>
-
  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Acknowledgement">Acknowledgement</a></li>
+
          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Acknowledgement">Acknowledgement</a></li>
-
  </ul>
+
        </ul>
-
              </li>
+
            </li>
-
  <li class="dropdown">
+
      <li class="dropdown"><a  href="https://2013.igem.org/Team:OUC-China/Safety">Safety</a></li>
-
                <a  href="https://2013.igem.org/Team:OUC-China/Safety"><font size="4.5px">Safety</font></a>
+
      <li class="dropdown">
-
               
+
                 <a class="dropdown-toggle" data-toggle="dropdown" href="#">Notebook<b class="caret"></b></a>
-
              </li>
+
                 <ul id="menu2" class="dropdown-menu" role="menu" aria-labelledby="dropdownMenu">
-
  <li class="dropdown">
+
-
                 <a class="dropdown-toggle" id="drop5" role="button" data-toggle="dropdown" href="#"><font size="4.5px">Notebook</font><b class="caret"></b></a>
+
-
                 <ul id="menu2" class="dropdown-menu" role="menu" aria-labelledby="drop5">
+
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Lab note">Lab note</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Lab note">Lab note</a></li>
-
                  <li role="presentation" class="divider"></li>
 
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Protocol">Protocol</a></li>
                   <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Protocol">Protocol</a></li>
                 </ul>
                 </ul>
-
              </li>
+
            </li>
-
  </ul>
+
    </ul>
-
</div>
+
  </div>
-
          </div>
+
</div>  
-
        </div>
+
-
      </div>
+
-
    </div>
+
-
<br><br>
+
-
 
+
<div class="container">
<div class="container">
-
 
<div class="row">
<div class="row">
-
       <div class="span3 bs-docs-sidebar">
+
       <div class="col-md-3 bs-docs-sidebar">
         <ul class="nav nav-list bs-docs-sidenav">
         <ul class="nav nav-list bs-docs-sidenav">
           <li><a href="#Introduction"><i class="icon-chevron-right"></i>Introduction</a></li>
           <li><a href="#Introduction"><i class="icon-chevron-right"></i>Introduction</a></li>
           <li><a href="https://2013.igem.org/Team:OUC-China/RNA guardian/Design"><i class="icon-chevron-right"></i>Design</a></li>
           <li><a href="https://2013.igem.org/Team:OUC-China/RNA guardian/Design"><i class="icon-chevron-right"></i>Design</a></li>
           <li><a href="https://2013.igem.org/Team:OUC-China/RNA guardian/Results"><i class="icon-chevron-right"></i>Results</a></li>
           <li><a href="https://2013.igem.org/Team:OUC-China/RNA guardian/Results"><i class="icon-chevron-right"></i>Results</a></li>
-
  <li><a href="https://2013.igem.org/Team:OUC-China/Reference"><i class="icon-chevron-right"></i>Reference</a></li>
+
      <li><a href="https://2013.igem.org/Team:OUC-China/Reference"><i class="icon-chevron-right"></i>Reference</a></li>
            
            
         </ul>
         </ul>
       </div>
       </div>
-
 
+
       <div class="col-md-9">
-
       <div class="span9">
+
  <section id="Overview">
-
+
           <div>In China, there is an old proverb” increase income and reduce expenditure”. There are many ways to increase the expression efficiency of a gene, such as choose a stronger promoter or RBS. Changing the Promoter and RBS is just like “increasing income”. But it is more cost-efficient to increase the expression by reducing expenditure. So, can we increase the expression of a gene by stabilizing its mRNA?<br /><br />In many prokaryotes, We find that RNase E mediated RNA degradation is a main progress in mRNA regulation of RNA level. So, can we stabilize an mRNA by decreasing the degradation?
-
 
+
Previous studies revealed that the most important endonuclease for mRNA turnover in E. coli is RNase E. So, if we can inhibit its activity, then our goals would be achieved. It has been reported that mRNA is more stable when it is being translated and they explain it by the ribosome preventing mRNA degradation.<br /><br /><img src="https://static.igem.org/mediawiki/2013/7/71/Ouc-Instruction1.jpg" height="500" width="600"  /><br /><br />Fig.1. 5’-end-dependent mRNA decay in bacteria that contain the endonuclease RNase E or ahomolog thereof. Pyrophosphate removal by RppH generates a 5’-terminal monophosphatethat binds to a discrete pocket on the surface of RNase E, thereby facilitating mRNA cleavage at a downstream location by the active site of that enzyme. In E. coli, RNase E cleavage of primary transcripts can also occur by an alternative, 5’-end-independent mechanism that does not require prior pyrophosphate removal.<br /><br />So, an idea comes to our mind. Can the ribosome work as a barrier, preventing the activation of this enzyme? This is what we have tried to do.</p>
-
  <section id="Introduction">
+
  </section>
-
 
+
-
  <div class="page-header">
+
-
            <h1>Introduction</h1>
+
-
           </div>
+
-
  <div class="row">
+
-
 
+
-
  <div class="span9">
+
-
    <div class="row">
+
-
     
+
-
      <div class="span9"><p style="font-weight:normal;"><font size="2px">In China, there is an old proverb” increase income and reduce expenditure”. There are many ways to increase the expression efficiency of a gene, such as choose a stronger promoter or RBS. Changing the Promoter and RBS is just like “increasing income”. But it is more cost-efficient to increase the expression by reducing expenditure. So, can we increase the expression of a gene by stabilizing its mRNA?<br /><br />In many prokaryotes, We find that RNase E mediated RNA degradation is a main progress in mRNA regulation of RNA level. So, can we stabilize an mRNA by decreasing the degradation?
+
-
Previous studies revealed that the most important endonuclease for mRNA turnover in E. coli is RNase E. So, if we can inhibit its activity, then our goals would be achieved. It has been reported that mRNA is more stable when it is being translated and they explain it by the ribosome preventing mRNA degradation.<br /><br /><img src="https://static.igem.org/mediawiki/2013/7/71/Ouc-Instruction1.jpg" height="500" width="600"  /><br /><br />Fig.1. 5’-end-dependent mRNA decay in bacteria that contain the endonuclease RNase E or ahomolog thereof. Pyrophosphate removal by RppH generates a 5’-terminal monophosphatethat binds to a discrete pocket on the surface of RNase E, thereby facilitating mRNA cleavage at a downstream location by the active site of that enzyme. In E. coli, RNase E cleavage of primary transcripts can also occur by an alternative, 5’-end-independent mechanism that does not require prior pyrophosphate removal.<br /><br />So, an idea comes to our mind. Can the ribosome work as a barrier, preventing the activation of this enzyme? This is what we have tried to do.</font></p>
+
-
 
+
-
 
+
-
 
+
-
  <br/>
+
-
  </div>
+
-
    </div>
+
-
  </div>
+
-
</div><br /><br /></br>
+
-
+
-
    </section>
+
    </div>
    </div>
  </div>
  </div>
  </div>
  </div>
-
 
+
<!--footer-->
-
 
+
<footer>
-
 
+
<div class="container">
 +
<div class="row">
 +
<div class="col-md-1"></div>
 +
<div class="col-md-6 footerleft">
 +
  <h3 class="footer-title" style="color:#bdc1c5;">About</h3>
 +
  <p>Thanks to:</p>
 +
  <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Bootstrap&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Flat-ui&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Unslider</p>
 +
  <p>Designed and built by <a href="http://copypeng.com">@PengYong</a> and <a href="http://www.renren.com/345094399/profile?ref=opensearch_normal#notice">@ZhenYuchen.</a></p>
 +
  <p>Code licensed under Apache License v2.0</p>
 +
</div>
 +
<div class="col-md-4">
 +
  <div class="footer-banner">
 +
    <h3 class="footer-title">Contact US</h3>
 +
    <ul>
 +
      <li>E-mail:<a href="mailto:oucigem@163.com"> oucigem@163.com</a></li>
 +
      <li><a href="http://oucast.com"> Official website</a></li>
 +
      <li><a href="http://www.renren.com/oast"> Find us on Renren</a></li>
 +
      <li><a href="http://weibo.com/u/2805858363"> Find us on Weibo</a></li>
 +
      <li><a href="https://www.google.com/maps?q=+36.062884,+120.335542&sll=36.1360706,120.3648376&sspn=0.36606958894334163,0.9386959091014536&t=m&z=16">Find us on Google Map</a></li>
 +
    </ul>
 +
  </div>
 +
</div>
 +
<div class="col-md-1"></div>
 +
</div>
 +
</div>
 +
</footer>
<script src="http://code.jquery.com/jquery.js"></script>
<script src="http://code.jquery.com/jquery.js"></script>
-
<script src="http://oucast.com/igem/js/bootstrap.min.js"></script>
+
<script src="http://netdna.bootstrapcdn.com/bootstrap/3.0.0/js/bootstrap.min.js"></script>
-
<script src="http://oucast.com/igem/js/ouc2.js"></script>
+
-
<script src="http://oucast.com/igem/js/application.js"></script>
+
-
<script>
+
-
 
+
-
      !function ($) {
+
-
        $(function(){
+
-
          // carousel demo
+
-
          $('#myCarousel').carousel()
+
-
        })
+
-
      }(window.jQuery)
+
-
    </script>
+
-
<script src="http://oucast.com/igem/js/holder.js"></script>
+
</body>
</body>
</html>
</html>

Revision as of 04:05, 28 September 2013

Overview

In China, there is an old proverb” increase income and reduce expenditure”. There are many ways to increase the expression efficiency of a gene, such as choose a stronger promoter or RBS. Changing the Promoter and RBS is just like “increasing income”. But it is more cost-efficient to increase the expression by reducing expenditure. So, can we increase the expression of a gene by stabilizing its mRNA?

In many prokaryotes, We find that RNase E mediated RNA degradation is a main progress in mRNA regulation of RNA level. So, can we stabilize an mRNA by decreasing the degradation? Previous studies revealed that the most important endonuclease for mRNA turnover in E. coli is RNase E. So, if we can inhibit its activity, then our goals would be achieved. It has been reported that mRNA is more stable when it is being translated and they explain it by the ribosome preventing mRNA degradation.



Fig.1. 5’-end-dependent mRNA decay in bacteria that contain the endonuclease RNase E or ahomolog thereof. Pyrophosphate removal by RppH generates a 5’-terminal monophosphatethat binds to a discrete pocket on the surface of RNase E, thereby facilitating mRNA cleavage at a downstream location by the active site of that enzyme. In E. coli, RNase E cleavage of primary transcripts can also occur by an alternative, 5’-end-independent mechanism that does not require prior pyrophosphate removal.

So, an idea comes to our mind. Can the ribosome work as a barrier, preventing the activation of this enzyme? This is what we have tried to do.

Retrieved from "http://2013.igem.org/Team:OUC-China/Introduction"