Team:Paris Saclay/Notebook/August/1

From 2013.igem.org

(Difference between revisions)
(→1 - Gibson assembly.)
Line 15: Line 15:
Protocol : [[Team:Paris_Saclay/Protocols/Gibson|PCR_clean_up]]
Protocol : [[Team:Paris_Saclay/Protocols/Gibson|PCR_clean_up]]
-
 
+
Sample Volume:
-
Adding amount:
+
{|
{|

Revision as of 06:26, 12 August 2013

Navigation Home Team Project Labwork Notebook Human practices

Contents

Notebook : August 1

Lab work

A - Aerobic/Anaerobic regulation system / B - PCB sensing system

1 - Gibson assembly.

  • BphR2_part1, BphR2_part2 and plasmid PSB1C3
  • FNR_part1, FNR part1 and plasmid PSB1C3
  • RBS_FNR part1 and FNR_part2 and plasmid PSB1C3

Protocol : PCR_clean_up

Sample Volume:

  • Epperdorf 1 : BphR2 part1(1ul), BphR2 part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)
  • Epperdorf 2 : FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)
  • Epperdorf 3 : RBS_FNR part1(1ul), FNR part2(1ul) , plasmid PSB1C3(2ul),Water(1ul),MIX Gibson (15 ul)


Retrieved from "http://2013.igem.org/Team:Paris_Saclay/Notebook/August/1"