Team:Paris Saclay/Notebook/August/20

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Contents

1 Notebook : August 20
- 1.1 Lab work
  - 1.1.1 A - Aerobic/Anaerobic regulation system
    - 1.1.1.1 Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006
    - 1.1.1.2 1 - Sequences analysis
  - 1.1.2 A - Aerobic/Anaerobic regulation system / B - PCB sensor system

Notebook : August 20

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006

1 - Sequences analysis

Damir, XiaoJing

Sequencies were good. We obtain : BBa_K1155004, BBa_K1155005, BBa_K1155006.

A - Aerobic/Anaerobic regulation system / B - PCB sensor system

Objective : obtaining FNR and BphR2 proteins

1 - Electrophoresis of PCR products : BphR2 Part I

Nadia

Well 1 : 6µL DNA Ladder
Well 2 : 40µL BphR2 Part I +8µl of 6X loading dye
Gel : 0.8%

Expected sizes :

BphR2 Part I : 178bp

We obtain a frangment at the right size. We can purify it.

2 - Gel purification of PCR products : BphR2 Part I

Damir, Nadia

Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]

We lost our fragment. We will do the PCR again.

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