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Team:Paris Saclay/Notebook/August/7
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==='''A - Aerobic/Anaerobic regulation system'''=== | ==='''A - Aerobic/Anaerobic regulation system'''=== | ||
| - | ===='''Objective : obtaining | + | ===='''Objective : obtaining BBa_K1155007'''==== |
===='''1 - Ligation of RBS-LacZ-Term in PSB1C3 ==== | ===='''1 - Ligation of RBS-LacZ-Term in PSB1C3 ==== | ||
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* H2O : 3.5µL | * H2O : 3.5µL | ||
| - | ===='''2 - Tranformation | + | ===='''2 - Tranformation BBa_K115507 in DH5α==== |
XiaoJing | XiaoJing | ||
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Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]] | Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]] | ||
| - | ===='''Objective : characterize | + | ===='''Objective : characterize BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006'''==== |
===='''1 - Tranduction of Km in MG1655Z1 ==== | ===='''1 - Tranduction of Km in MG1655Z1 ==== | ||
Revision as of 09:23, 30 September 2013
Contents |
Notebook : August 7
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155007
1 - Ligation of RBS-LacZ-Term in PSB1C3
Anaïs, Nadia
Used quantities :
- RBS-AmilCP : 2µL
- Term in PSB1C3 : 2.5µL
- Buffer : 1µL
- Ligase : 1µL
- H2O : 3.5µL
2 - Tranformation BBa_K115507 in DH5α
XiaoJing
Protocol : Bacterial transformation
Objective : characterize BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006
1 - Tranduction of Km in MG1655Z1
Nadia, XioaJing
Protocol : Transduction
Our Mutant bacteria was called BW : Δfnr::Km. Our wild type bacteria was called MG1655Z1.
We did the first step of the protocol : bacteriophage stock which packed Km gene.
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