Notebook : August 7

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Bba_K1155007

1 - Ligation of RBS-LacZ-Term in PSB1C3

Anaïs, Nadia

Used quantities :

• RBS-AmilCP : 2µL
• Term in PSB1C3 : 2.5µL
• Buffer : 1µL
• Ligase : 1µL
• H2O : 3.5µL

2 - Tranformation Bba_K115507 in DH5α

XiaoJing

Protocol : Bacterial transformation

Objective : characterize Bba_K1155000, Bba_K1155004, Bba_K1155005, Bba_K1155006

1 - Tranduction of Km in MG1655Z1

Nadia, XioaJing

Protocol : Transduction

Our Mutant bacteria was called BW : Δfnr::Km. Our wild type bacteria was called MG1655Z1.

We did the first step of the protocol : bacteriophage stock which packed Km gene.