Team:Paris Saclay/Notebook/July/4

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Notebook : July 4

Summary:

For regulator system:
  • estimated the size of segments for bands of electrophoresis by using Clone Manager.
  • Made interpretation for electrophoresis. The picture shows that the experimental result was coherent with our estimation. We constructed our first BrioBrick, BBa_K1155000 (fnr+plasmid PSB1C3).
  • stored 2 colonies who contain BioBrick BBa_K1155000
  • the plasmid DNA extraction was performed for BBa_K1155000.
  • The extract of plasmid DNA which contain BBa_K1155000 was digested by Not I, Mlu I, Hpa I, and were amplified by PCR.
  • Seeded the 4 additional broths (2 for amilCP, 2 for FNR) for plasmid extraction(mini prep).

For PCBs sensor system:

  • Received the bacterial strain: pseudomonas KE707.

Lab work

constructing



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