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Team:Paris Saclay/Notebook/July/5
From 2013.igem.org
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Transformation of 07/04 works. We will extract DNA plasmid. | Transformation of 07/04 works. We will extract DNA plasmid. | ||
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| - | Protocol : [[Team:Paris_Saclay/extraction| | + | Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]] |
Nanodrop : | Nanodrop : | ||
Revision as of 13:19, 25 September 2013
Contents |
Notebook : July 5
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155000
1 - Electrophoresis of the digestion of Bba_K1155000 by NotI, HpaI, MluI
Sheng, Zhou
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Expected sizes :
- Bba_K1155000 : ...
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We didn't obtain fragments at the right size. We will do digestions again. |
2 - Extraction of Bba_K1155000 and Bba_K592009 from DH5α
Abdou
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Transformation of 07/04 works. We will extract DNA plasmid. |
Protocol : High copy plamid extraction
Nanodrop :
- Bba_K1155000P : 761ng/µl
- Bba_K592009 : 229ng/µl
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By using NanoDrop, we found some errors which could be due to device malfunctional issue. |
Electrophoresis band size estimation
We used Clonemanager for band size estimation:
| Molecule | enzymes(Buffer) | Size |
| AmilCP clone | Nod I(Orange) | 2046+693bp |
| AmilCP clone | XHOI(Red) | 1842+892bp |
| BBa_K1155000 | XHOI(Red) | 1289+892bp |
| BBa_K1155000 | ECoR I(Orange) | 2151bp |
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