Team:Paris Saclay/Notebook/July/5
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='''Notebook : July 5'''= | ='''Notebook : July 5'''= | ||
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=='''Lab work'''== | =='''Lab work'''== | ||
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==='''A - Aerobic/Anaerobic regulation system'''=== | ==='''A - Aerobic/Anaerobic regulation system'''=== | ||
- | ===='''Objective : obtaining | + | ===='''Objective : obtaining BBa_K1155000'''==== |
- | ===='''1 - Electrophoresis of the digestion of | + | ===='''1 - Electrophoresis of the digestion of BBa_K1155000 by NotI, HpaI, MluI'''==== |
Sheng, Zhou | Sheng, Zhou | ||
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| style="width:350px;border:1px solid black;" | [[File:PCRPS050813.jpg|right|350px]] | | style="width:350px;border:1px solid black;" | [[File:PCRPS050813.jpg|right|350px]] | ||
| style="width:350px;border:1px solid black;" | | | style="width:350px;border:1px solid black;" | | ||
- | * Well 1, 2 : 5µL of | + | * Well 1, 2 : 5µL of BBa_K1155000 digested by Not I+1µL of 6X loading dye |
- | * Well 3, 4 : 5µL of | + | * Well 3, 4 : 5µL of BBa_K1155000 digested by Hpa I+1µL of 6X loading dye |
* Well 5 : 6µL of DNA Ladder | * Well 5 : 6µL of DNA Ladder | ||
- | * Well 6, 7 : 5µL of | + | * Well 6, 7 : 5µL of BBa_K1155000 digested by Mlu I+1µL of 6X loading dye |
* Gel 0.8% | * Gel 0.8% | ||
|} | |} | ||
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+ | Expected sizes : | ||
+ | * BBa_K1155000 : 111bp | ||
{| | {| | ||
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|} | |} | ||
- | ===='''2 - Extraction of | + | ===='''2 - Extraction of BBa_K1155000 from DH5α'''==== |
Abdou | Abdou | ||
+ | {| | ||
+ | | style="border:1px solid black;padding:5px;background-color:#DE;" | | ||
+ | Transformation of 07/04 works. We will extract DNA plasmid. | ||
+ | |} | ||
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+ | Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]] | ||
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+ | Nanodrop : | ||
+ | * BBa_K1155000 : 761ng/µl | ||
+ | |||
+ | {| | ||
+ | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
+ | By using NanoDrop, we found some errors which could be due to device malfunctional issue. | ||
+ | |} | ||
+ | |||
+ | ===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''==== | ||
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+ | ===='''1 - Extraction of BBa_K592009 and BBa_I732017 from DH5α'''==== | ||
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+ | Abdou | ||
{| | {| | ||
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Transformation of 07/04 works. We will extract DNA plasmid. | Transformation of 07/04 works. We will extract DNA plasmid. | ||
|} | |} | ||
- | Protocol : [[Team:Paris_Saclay/ | + | Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]] |
Nanodrop : | Nanodrop : | ||
- | * | + | * BBa_K592009 : 229ng/µl |
- | + | ||
{| | {| | ||
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By using NanoDrop, we found some errors which could be due to device malfunctional issue. | By using NanoDrop, we found some errors which could be due to device malfunctional issue. | ||
|} | |} | ||
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{| border="1" align="center" | {| border="1" align="center" |
Latest revision as of 16:35, 4 October 2013
Contents |
Notebook : July 5
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155000
1 - Electrophoresis of the digestion of BBa_K1155000 by NotI, HpaI, MluI
Sheng, Zhou
|
Expected sizes :
- BBa_K1155000 : 111bp
We didn't obtain fragments at the right size. We will do digestions again. |
2 - Extraction of BBa_K1155000 from DH5α
Abdou
Transformation of 07/04 works. We will extract DNA plasmid. |
Protocol : High copy plamid extraction
Nanodrop :
- BBa_K1155000 : 761ng/µl
By using NanoDrop, we found some errors which could be due to device malfunctional issue. |
Objective : obtaining BBa_K1155003, BBa_K1155007
1 - Extraction of BBa_K592009 and BBa_I732017 from DH5α
Abdou
Transformation of 07/04 works. We will extract DNA plasmid. |
Protocol : High copy plamid extraction
Nanodrop :
- BBa_K592009 : 229ng/µl
By using NanoDrop, we found some errors which could be due to device malfunctional issue. |
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