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Team:Paris Saclay/Notebook/July/5
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==='''A - Aerobic/Anaerobic regulation system'''=== | ==='''A - Aerobic/Anaerobic regulation system'''=== | ||
| - | ===='''Objective : obtaining | + | ===='''Objective : obtaining BBa_K1155000'''==== |
| - | ===='''1 - Electrophoresis of the digestion of | + | ===='''1 - Electrophoresis of the digestion of BBa_K1155000 by NotI, HpaI, MluI'''==== |
Sheng, Zhou | Sheng, Zhou | ||
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| style="width:350px;border:1px solid black;" | [[File:PCRPS050813.jpg|right|350px]] | | style="width:350px;border:1px solid black;" | [[File:PCRPS050813.jpg|right|350px]] | ||
| style="width:350px;border:1px solid black;" | | | style="width:350px;border:1px solid black;" | | ||
| - | * Well 1, 2 : 5µL of | + | * Well 1, 2 : 5µL of BBa_K1155000 digested by Not I+1µL of 6X loading dye |
| - | * Well 3, 4 : 5µL of | + | * Well 3, 4 : 5µL of BBa_K1155000 digested by Hpa I+1µL of 6X loading dye |
* Well 5 : 6µL of DNA Ladder | * Well 5 : 6µL of DNA Ladder | ||
| - | * Well 6, 7 : 5µL of | + | * Well 6, 7 : 5µL of BBa_K1155000 digested by Mlu I+1µL of 6X loading dye |
* Gel 0.8% | * Gel 0.8% | ||
|} | |} | ||
Expected sizes : | Expected sizes : | ||
| - | * | + | * BBa_K1155000 : 111bp |
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| - | ===='''2 - Extraction of | + | ===='''2 - Extraction of BBa_K1155000 from DH5α'''==== |
Abdou | Abdou | ||
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Nanodrop : | Nanodrop : | ||
| - | * | + | * BBa_K1155000 : 761ng/µl |
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| - | ===='''Objective : obtaining | + | ===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''==== |
| - | ===='''1 - Extraction of | + | ===='''1 - Extraction of BBa_K592009 and BBa_I732017 from DH5α'''==== |
Abdou | Abdou | ||
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Nanodrop : | Nanodrop : | ||
| - | * | + | * BBa_K592009 : 229ng/µl |
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Latest revision as of 16:35, 4 October 2013
Contents |
Notebook : July 5
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155000
1 - Electrophoresis of the digestion of BBa_K1155000 by NotI, HpaI, MluI
Sheng, Zhou
 |
|
Expected sizes :
- BBa_K1155000 : 111bp
|
We didn't obtain fragments at the right size. We will do digestions again. |
2 - Extraction of BBa_K1155000 from DH5α
Abdou
|
Transformation of 07/04 works. We will extract DNA plasmid. |
Protocol : High copy plamid extraction
Nanodrop :
- BBa_K1155000 : 761ng/µl
|
By using NanoDrop, we found some errors which could be due to device malfunctional issue. |
Objective : obtaining BBa_K1155003, BBa_K1155007
1 - Extraction of BBa_K592009 and BBa_I732017 from DH5α
Abdou
|
Transformation of 07/04 works. We will extract DNA plasmid. |
Protocol : High copy plamid extraction
Nanodrop :
- BBa_K592009 : 229ng/µl
|
By using NanoDrop, we found some errors which could be due to device malfunctional issue. |
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