Team:Paris Saclay/Notebook/July/5
From 2013.igem.org
Contents |
Notebook : July 5
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155000
1 - Electrophoresis of the digestion of Bba_K1155000 by NotI, HpaI, MluI
Sheng, Zhou
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Expected sizes :
- Bba_K1155000 : ...
We didn't obtain fragments at the right size. We will do digestions again. |
2 - Extraction of Bba_K1155000 from DH5α
Abdou
Transformation of 07/04 works. We will extract DNA plasmid. |
Protocol : High copy plamid extraction
Nanodrop :
- Bba_K1155000 : 761ng/µl
By using NanoDrop, we found some errors which could be due to device malfunctional issue. |
Objective : obtaining Bba_K1155003, BBa_K1155007
1 - Extraction of Bba_K592009 and Bba_I732017 from DH5α
Abdou
Transformation of 07/04 works. We will extract DNA plasmid. |
Protocol : High copy plamid extraction
Nanodrop :
- Bba_K592009 : 229ng/µl
By using NanoDrop, we found some errors which could be due to device malfunctional issue. |
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