Ethanol precipitation

This method is used to purify and/or concentrate DNA, RNA and polysaccarides.

Add in a volume of DNA :

1. 3 volumes of ethanol 100%

2. 1/10 of volume of sodium acetate 3M

3. 1µL of glycogen

4. Let the solution at -20°C during 30min

5. Centrifuge at maximal speed during 30min at 4°C

6. Put away the supernatant

7. Wash with 1mL of ethanol 70%

8. Centrifuge 10-15 min at 4°C

9. Put away the supernatant, dry (speed vacuum 4 min)

10. Repeat with water or TE