Team:TMU-Tokyo/Notebook experiment/1st

From 2013.igem.org

(Difference between revisions)
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DNA Purification following fragment from gel.<Br>
DNA Purification following fragment from gel.<Br>
<Table>
<Table>
-
<Tr><Td>PtetR1</Td></Tr>
+
<Tr><Td>PtetR1, FLP, Sm, araC-P, hbiF, Tc, lacI, FRT-Cm, FRT-Ap, Km-Blunt</Td></Tr>
-
<Tr><Td>FLP</Td></Tr>
+
-
<Tr><Td>Sm</Td></Tr>
+
-
<Tr><Td>araC-P</Td></Tr>
+
-
<Tr><Td>hbiF</Td></Tr>
+
-
<Tr><Td>Tc</Td></Tr>
+
-
<Tr><Td>lacI</Td></Tr>
+
-
<Tr><Td>FRT-Cm</Td></Tr>
+
-
<Tr><Td>FRT-Ap</Td></Tr>
+
-
<Tr><Td>Km-Blunt</Td></Tr>
+
</Table>
</Table>
<Br>
<Br>
<Br>
<Br>
<A href="https://2013.igem.org/Team:TMU-Tokyo/Notebook_experiment/2st">>>Go to next week</A>
<A href="https://2013.igem.org/Team:TMU-Tokyo/Notebook_experiment/2st">>>Go to next week</A>

Revision as of 16:15, 27 September 2013



Experiment > Parts Synthesis > 1st week



September 2nd
PCR following fragment.
FragmentPtetR1PtetR2FLPSmaraC-P
PrimerCmNR-stu
FLP-IRR/IRL
CmCR-stu
FLP-IRR/IRL
FLP-L
FLP-R
FLP-SaApL
CmC
acaC-2
BAD-C3
TempleteIRR/IRL-GFP
(BBa_K880002)
IRR/IRL-GFP
(BBa_K880002)
pFTGTm(C2-Ap)SmpBAD/Myc-HisA
FragmenthbiFTclacIFRT-CmFRT-Ap
PrimerpBAD-HbiF
RTcN-hbiF
TcN
TcC3
141-70
FRT-lacIC
R6Kori-Xho-3R
R(FRT)2
lacI-R(FRT)2
mhpA-SaApR
TempletehbiF
(BBa_K880000)
Escherichia coli
MG1655 recA⁻
(cell suspention)
Escherichia coli
MG1655 genome
pSG76CpSG76A2

Result

1: PterR1, 2: PtetR2, 3: FLP, 4: Sm, 5: araC-P,
6: hbiF, 7: Tc, 8: LacI, 9: FRT-Cm, 10: FRT-Ap


September 3rd
Vector Remodeling with PCR. Insertion Recognition sites (Blunt).
FragmentKm-Blunt
PrimerKm-Blunt-N
Km-Blunt-C
TempletepACYC177

Result



DNA Purification following fragment from gel.
PtetR1, FLP, Sm, araC-P, hbiF, Tc, lacI, FRT-Cm, FRT-Ap, Km-Blunt


>>Go to next week