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Team:TU-Delft/Notebook/2013/07/08/
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3. Analysis of the Gel electrophoresis in lab meeting. pBAD and pT7 was not that good. AIP sender and receiver also shows contamination of wells. <br> | 3. Analysis of the Gel electrophoresis in lab meeting. pBAD and pT7 was not that good. AIP sender and receiver also shows contamination of wells. <br> | ||
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| + | 4. The biobricks: pBAD, pT7, GFP, AIP sender(Agr A: AgrC). AIP receiver(AgrB: AgrD), pTET:lysis were grown on Agar plates. But the negative control is not convincing, so Gel electrophoresis is run on random colonies from the plates to check. <br> | ||
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Revision as of 11:05, 30 July 2013
Notebook
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4th of July
Lab work
1. Biobricks from the iGEM registry were streaked on Agar plates.
2. Transformation of the pTET backbone with Lysis cassette, which was done on 5/7/13.
3. Analysis of the Gel electrophoresis in lab meeting. pBAD and pT7 was not that good. AIP sender and receiver also shows contamination of wells.
4. The biobricks: pBAD, pT7, GFP, AIP sender(Agr A: AgrC). AIP receiver(AgrB: AgrD), pTET:lysis were grown on Agar plates. But the negative control is not convincing, so Gel electrophoresis is run on random colonies from the plates to check.
