Team:TU-Delft/Notebook/2013/07/10/
From 2013.igem.org
(Difference between revisions)
(2 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
{{:Team:TU-Delft/Templates/Navigation}} | {{:Team:TU-Delft/Templates/Navigation}} | ||
{{:Team:TU-Delft/Templates/Style}} | {{:Team:TU-Delft/Templates/Style}} | ||
+ | {{:Team:TU-Delft/Templates/Frog}} | ||
+ | {{:Team:TU-Delft/Templates/Logo}} | ||
{{:Team:TU-Delft/Templates/LabNotes}} | {{:Team:TU-Delft/Templates/LabNotes}} | ||
+ | |||
<html> | <html> | ||
</br></br></br></br></br></br> | </br></br></br></br></br></br> | ||
+ | <a name="lab8"></a> | ||
</html> | </html> | ||
Line 43: | Line 47: | ||
<td>DH5Alpha</td> | <td>DH5Alpha</td> | ||
</tr> | </tr> | ||
- | </table | + | </table><br>All transformations were streaked on agar plates with corresponding antibiotic resistance.<br><br> |
5. Prepared flasks for autoclaving. <br> | 5. Prepared flasks for autoclaving. <br> | ||
</p> | </p> |
Latest revision as of 08:00, 4 October 2013
Notebook
Sun | Mon | Tue | Wed | Thu | Fri | Sat |
---|---|---|---|---|---|---|
1 | ||||||
2 | 3 | 4 | 5 | 6 | 7 | 8 |
9 | 10 | 11 | 12 | 13 | 14 | 15 |
16 | 17 | 18 | 19 | 20 | 21 | 22 |
23 | 24 | 25 | 26 | 27 | 28 | 29 |
30 |
Sun | Mon | Tue | Wed | Thu | Fri | Sat |
---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | |
7 | 8 | 9 | 10 | 11 | 12 | 13 |
14 | 15 | 16 | 17 | 18 | 19 | 20 |
21 | 22 | 23 | 24 | 25 | 26 | 27 |
28 | 29 | 30 | 31 |
Sun | Mon | Tue | Wed | Thu | Fri | Sat |
---|---|---|---|---|---|---|
1 | 2 | 3 | ||||
4 | 5 | 6 | 7 | 8 | 9 | 10 |
11 | 12 | 13 | 14 | 15 | 16 | 17 |
18 | 19 | 20 | 21 | 22 | 23 | 24 |
25 | 26 | 27 | 28 | 29 | 30 | 31 |
Sun | Mon | Tue | Wed | Thu | Fri | Sat |
---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 |
8 | 9 | 10 | 11 | 12 | 13 | 14 |
15 | 16 | 17 | 18 | 19 | 20 | 21 |
22 | 23 | 24 | 25 | 26 | 27 | 28 |
29 | 30 |
10th of July
Lab work
1. Made glycerol stocks for all the transformations done on 9/7/13.
2. DNA extraction of all transformations to send for sequencing.
3. Experiment with tetracycline for checking of pTET:lysis device failed.
4. Started new transformations :
Part | Resistance | Cells |
pTET:lysis device | Ampicillin | DH5Alpha |
AgrB:AgrD | Kanamycin | BL21 |
pConst | Ampicillin | DH5Alpha |
All transformations were streaked on agar plates with corresponding antibiotic resistance.
5. Prepared flasks for autoclaving.