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Team:TU-Delft/Notebook/2013/07/12/
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| - | <p> 1. Gel electrophoresis <br> | + | <p> 1. Gel electrophoresis on the restriction digested parts were carried out today.<br> |
| - | 2. | + | 2. Gel extraction was done. <br> |
| - | 3. Restriction digestion was | + | 3. Restriction digestion of lysis device having the prefix and suffix with XbaI +PstI +DpnI was done, and ligated to pTET which was cut with SpeI +PstI. As the lysis device and backbone are of the same size, the ligation will not be successful. So we use Dpn I to digest the E.coli made methylated DNA while doing the PCR. <br> |
| - | + | ||
</p> | </p> | ||
Revision as of 09:56, 31 July 2013
Notebook
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12th of July
Lab work
1. Gel electrophoresis on the restriction digested parts were carried out today.
2. Gel extraction was done.
3. Restriction digestion of lysis device having the prefix and suffix with XbaI +PstI +DpnI was done, and ligated to pTET which was cut with SpeI +PstI. As the lysis device and backbone are of the same size, the ligation will not be successful. So we use Dpn I to digest the E.coli made methylated DNA while doing the PCR.
