"
Team:TU-Delft/Notebook/2013/07/17/
From 2013.igem.org
(Difference between revisions)
(Created page with "{{:Team:TU-Delft/Templates/Navigation}} {{:Team:TU-Delft/Templates/Style}} {{:Team:TU-Delft/Templates/LabNotes}} <html> </br></br></br></br></br></br> </html> <br><br><br><br> ...") |
|||
| (3 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{:Team:TU-Delft/Templates/Navigation}} | {{:Team:TU-Delft/Templates/Navigation}} | ||
{{:Team:TU-Delft/Templates/Style}} | {{:Team:TU-Delft/Templates/Style}} | ||
| + | {{:Team:TU-Delft/Templates/Frog}} | ||
| + | {{:Team:TU-Delft/Templates/Logo}} | ||
{{:Team:TU-Delft/Templates/LabNotes}} | {{:Team:TU-Delft/Templates/LabNotes}} | ||
| + | |||
<html> | <html> | ||
</br></br></br></br></br></br> | </br></br></br></br></br></br> | ||
| + | <a name="lab15"></a> | ||
</html> | </html> | ||
| Line 18: | Line 22: | ||
<p> 1. AIP receiver:GFP and pTET:RBS:cI was ligated and grown in Agar plates, then cultured, DNA extracted. <br> | <p> 1. AIP receiver:GFP and pTET:RBS:cI was ligated and grown in Agar plates, then cultured, DNA extracted. <br> | ||
2. Running gel electrophoresis on the restriction digestion of AIP receiver:GFP with XbaI +PstI and pTET:RBS:cI with EcoRI +SpeI. <br> | 2. Running gel electrophoresis on the restriction digestion of AIP receiver:GFP with XbaI +PstI and pTET:RBS:cI with EcoRI +SpeI. <br> | ||
| - | 2. Checking for Quick change on SUMO and Ulp 1, which was not successful at PCR | + | 2. Checking for Quick change on SUMO and Ulp 1, which was not successful at PCR annealing temperature at 50 degree and 55 degree. <br> |
3. Transformation of pT7(BBa_I719005) in DH5Alpha cells was carried out. <br> | 3. Transformation of pT7(BBa_I719005) in DH5Alpha cells was carried out. <br> | ||
</p> | </p> | ||
Latest revision as of 08:27, 4 October 2013
Notebook
| Sun | Mon | Tue | Wed | Thu | Fri | Sat |
|---|---|---|---|---|---|---|
| 1 | ||||||
| 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| 9 | 10 | 11 | 12 | 13 | 14 | 15 |
| 16 | 17 | 18 | 19 | 20 | 21 | 22 |
| 23 | 24 | 25 | 26 | 27 | 28 | 29 |
| 30 |
| Sun | Mon | Tue | Wed | Thu | Fri | Sat |
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | |
| 7 | 8 | 9 | 10 | 11 | 12 | 13 |
| 14 | 15 | 16 | 17 | 18 | 19 | 20 |
| 21 | 22 | 23 | 24 | 25 | 26 | 27 |
| 28 | 29 | 30 | 31 |
| Sun | Mon | Tue | Wed | Thu | Fri | Sat |
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | ||||
| 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| 11 | 12 | 13 | 14 | 15 | 16 | 17 |
| 18 | 19 | 20 | 21 | 22 | 23 | 24 |
| 25 | 26 | 27 | 28 | 29 | 30 | 31 |
| Sun | Mon | Tue | Wed | Thu | Fri | Sat |
|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| 8 | 9 | 10 | 11 | 12 | 13 | 14 |
| 15 | 16 | 17 | 18 | 19 | 20 | 21 |
| 22 | 23 | 24 | 25 | 26 | 27 | 28 |
| 29 | 30 |
17th of July
Lab work
1. AIP receiver:GFP and pTET:RBS:cI was ligated and grown in Agar plates, then cultured, DNA extracted.
2. Running gel electrophoresis on the restriction digestion of AIP receiver:GFP with XbaI +PstI and pTET:RBS:cI with EcoRI +SpeI.
2. Checking for Quick change on SUMO and Ulp 1, which was not successful at PCR annealing temperature at 50 degree and 55 degree.
3. Transformation of pT7(BBa_I719005) in DH5Alpha cells was carried out.
