Team:TU-Delft/Notebook/2013/09/03/
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Notebook
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3rd of September
Lab work
1. The colony PCR was run on gel. Gel pictures are as follows:
2. The restriction digestion on gel :
3. As the restriction digestion gels were not that convincing, digestions were started again on :
Lysis device with E+X and X+P
pT7 with S+P
AIP receiver with X+P
pBAD with S+P
4. Ligations were carried out after gel extractions from today's gels. They were:
Lysis device (E+X) with pcI:Ulp (E+S)
(S+P) with Lysis device (X+P)
5. Started Restriction digestion on :
Lysis device(E+S)
RBS:TetR:TT (E+S) for the pSB1C3 backbone