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Team:TU-Delft/Notebook/2013/09/22/
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2. Colony PCR was done for the following: <br><br> | 2. Colony PCR was done for the following: <br><br> | ||
<left> | <left> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/e/e0/130922_pt7_sumo_peptide_bl21_plyss.jpg" height= | + | <img src="https://static.igem.org/mediawiki/2013/e/e0/130922_pt7_sumo_peptide_bl21_plyss.jpg" height=400px width="500"> |
</left><br><br> | </left><br><br> | ||
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| - | <img src="https://static.igem.org/mediawiki/2013/f/f2/130922_ptet_ci_tt_backbone_s%2Bp_pci_ulp_insert_x_%2B_p.jpg" height= | + | <img src="https://static.igem.org/mediawiki/2013/f/f2/130922_ptet_ci_tt_backbone_s%2Bp_pci_ulp_insert_x_%2B_p.jpg" height=400px width="500"> |
</left><br><br> | </left><br><br> | ||
4. Ligation of the pTet cI TT + pcI Ulp (Batch B). <br> | 4. Ligation of the pTet cI TT + pcI Ulp (Batch B). <br> | ||
| - | 5. Inoculated :<br> | + | 5. Inoculated :<br><br> |
pBAD Ulp TT pT7 SUMO Peptides in BL21 from the glycerol stocks. <br> | pBAD Ulp TT pT7 SUMO Peptides in BL21 from the glycerol stocks. <br> | ||
<i> S. delphini, B. subtilis,</i> and BL21 cells. <br> | <i> S. delphini, B. subtilis,</i> and BL21 cells. <br> | ||
Sender and receiver cassette <br> | Sender and receiver cassette <br> | ||
| - | GFP constitutive (for control) <br> | + | GFP constitutive (for control) <br><br> |
6. Restriction digestion of pTet cI TT pSB1C3 with S+P and pcI Ulp pSB1C3 with X+P was started. <br> | 6. Restriction digestion of pTet cI TT pSB1C3 with S+P and pcI Ulp pSB1C3 with X+P was started. <br> | ||
7. Sent all samples which were minipreped for sequencing. <br> | 7. Sent all samples which were minipreped for sequencing. <br> | ||
Revision as of 13:48, 27 September 2013
Notebook
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22nd of September
Lab work
1. Mini prep was done on the colonies grown from yesterday:
pBAD Ulp TT pT7 SUMO signiferin, pcI Ulp pSB1C3, Lysis pSB1C3, pTet cI TT pSB1C3
2. Colony PCR was done for the following:
3. Ran the restriction digestions on gel:
4. Ligation of the pTet cI TT + pcI Ulp (Batch B).
5. Inoculated :
pBAD Ulp TT pT7 SUMO Peptides in BL21 from the glycerol stocks.
S. delphini, B. subtilis, and BL21 cells.
Sender and receiver cassette
GFP constitutive (for control)
6. Restriction digestion of pTet cI TT pSB1C3 with S+P and pcI Ulp pSB1C3 with X+P was started.
7. Sent all samples which were minipreped for sequencing.
