Team:TU-Delft/Notebook/2013/09/25/
From 2013.igem.org
(Difference between revisions)
(One intermediate revision not shown) | |||
Line 8: | Line 8: | ||
<html> | <html> | ||
</br></br></br></br></br></br> | </br></br></br></br></br></br> | ||
+ | <a name="lab25"></a> | ||
</html> | </html> | ||
Line 34: | Line 35: | ||
Lysis pSB1C3 with E+S <br> | Lysis pSB1C3 with E+S <br> | ||
- | 3. | + | 3. Restriction digestions which were running was put on gel: <br> |
+ | <left> | ||
+ | <img src="https://static.igem.org/mediawiki/2013/e/e6/130925_RD2.jpg" height=400px width="600"> | ||
+ | </left><br> | ||
4. Sender receiver experiment was done with the existing construct, but it was a failure. <br> | 4. Sender receiver experiment was done with the existing construct, but it was a failure. <br> | ||
+ | |||
+ | 5. Colony PCR of the pBAD AIP receiver GFP was done but no bands were seen. This proved that the construct was faulty. <br> Ligation was started again to make the construct. <br> |
Latest revision as of 09:15, 4 October 2013
Notebook
Sun | Mon | Tue | Wed | Thu | Fri | Sat |
---|---|---|---|---|---|---|
1 | ||||||
2 | 3 | 4 | 5 | 6 | 7 | 8 |
9 | 10 | 11 | 12 | 13 | 14 | 15 |
16 | 17 | 18 | 19 | 20 | 21 | 22 |
23 | 24 | 25 | 26 | 27 | 28 | 29 |
30 |
Sun | Mon | Tue | Wed | Thu | Fri | Sat |
---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | |
7 | 8 | 9 | 10 | 11 | 12 | 13 |
14 | 15 | 16 | 17 | 18 | 19 | 20 |
21 | 22 | 23 | 24 | 25 | 26 | 27 |
28 | 29 | 30 | 31 |
Sun | Mon | Tue | Wed | Thu | Fri | Sat |
---|---|---|---|---|---|---|
1 | 2 | 3 | ||||
4 | 5 | 6 | 7 | 8 | 9 | 10 |
11 | 12 | 13 | 14 | 15 | 16 | 17 |
18 | 19 | 20 | 21 | 22 | 23 | 24 |
25 | 26 | 27 | 28 | 29 | 30 | 31 |
Sun | Mon | Tue | Wed | Thu | Fri | Sat |
---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 |
8 | 9 | 10 | 11 | 12 | 13 | 14 |
15 | 16 | 17 | 18 | 19 | 20 | 21 |
22 | 23 | 24 | 25 | 26 | 27 | 28 |
29 | 30 |
25th of September
Lab work
1. Colony PCR was done for the following:
Inoculated colonies 1 and 4.
2. Restriction digestions was started for :
pTet cI TT pcI Ulp (col 1) with X+P
Lysis pSB1C3 with S+P
BBa_I746008 (for pSB2K3) with E+S
Lysis pSB1C3 with E+S
3. Restriction digestions which were running was put on gel:
4. Sender receiver experiment was done with the existing construct, but it was a failure.
5. Colony PCR of the pBAD AIP receiver GFP was done but no bands were seen. This proved that the construct was faulty.
Ligation was started again to make the construct.