Team:TU Darmstadt/materials

From 2013.igem.org

(Difference between revisions)
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== Enzymes ==
== Enzymes ==
=== Phosphatases ===
=== Phosphatases ===
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Antarctic_Phosphatase Antarctic Phosphatase]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/Antarctic_Phosphatase Antarctic Phosphatase]
=== DNA polymerases ===
=== DNA polymerases ===
* [https://2013.igem.org/Team:TU_Darmstadt/materials/Phusion Phusion]
* [https://2013.igem.org/Team:TU_Darmstadt/materials/Phusion Phusion]
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* [https://2013.igem.org/Team:TU_Darmstadt/materials/InFusion_Pfu 5x InFusion Pfu MasterMix]
* [https://2013.igem.org/Team:TU_Darmstadt/materials/InFusion_Pfu 5x InFusion Pfu MasterMix]
=== DNA ligases ===
=== DNA ligases ===
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/T4_DNA_Ligase T4 DNA ligase]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/T4_DNA_Ligase T4 DNA ligase]
=== Restriction enzymes ===
=== Restriction enzymes ===
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/NheI NheI]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/NheI NheI]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/EcoRI EcoRI]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/EcoRI EcoRI]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/PstI PstI]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/PstI PstI]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/SpeI SpeI]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/SpeI SpeI]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/XbaI XbaI]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/XbaI XbaI]
== Electrophoresis ==
== Electrophoresis ==
=== Agarose gel electrophoresis ===
=== Agarose gel electrophoresis ===
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Agarose_gel_loading_buffer Agarose gel loading buffer]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/TriTrack_LoadingDye TriTrack loading dye]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Bromphenol_blue Bromphenol blue]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/DNA_ladder DNA ladders]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/DNA_ladder DNA ladder]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/Nancy Nancy]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Ethidium_Bromide Ethidium Bromide]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/TAE TAE]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Safe_stain Roti<sup>®</sup> SafeGelStain]
+
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/TAE TAE]
+
=== PAGE ===
=== PAGE ===
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Coomassie_stain Coomassie stain]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/Coomassie_stain Coomassie stain]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Destain Destain]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/Destain Destain]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/SDS-Page SDS-Page]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/SDS-Page SDS-Page]
== Buffers ==
== Buffers ==
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/PBS PBS]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/PBS PBS]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/TE TE]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/TE TE]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Tris Tris]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/Tris Tris]
== Induction ==
== Induction ==
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/Arabinose Arabinose]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/Arabinose Arabinose]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Materials/IPTG IPTG]
+
* [https://2013.igem.org/Team:TU_Darmstadt/materials/IPTG IPTG]
== DNA sequences ==
== DNA sequences ==

Revision as of 11:36, 3 October 2013

Labbook | Protocols | Materials |

This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the [http://openwetware.org/wiki/Materials OWW materials] page.

Can't find what you seek? Try their excellent page.

Contents

Strains

  • [http://ecoliwiki.net/colipedia/index.php/DH5_alpha DH5α]
  • [http://ecoliwiki.net/colipedia/index.php/TOP10 TOP10]
  • [http://ecoliwiki.net/colipedia/index.php/BL21(DE3) BL21DE3]

Media

Antibiotics

Enzymes

Phosphatases

DNA polymerases

DNA ligases

Restriction enzymes

Electrophoresis

Agarose gel electrophoresis

PAGE

Buffers

Induction

DNA sequences